Feeding enzymes to poultry is one of the major nutritional advances in the last fifty years. It is the culmination of something that nutritionists realized for a long time but until 1980’s it remained beyond their reach. Indeed, the theory of feed enzymes is simple. Plants contain some compounds that either the animal cannot digest, or which hinder its digestive system, often because the animal cannot produce the necessary enzyme to degrade them. Nutritionists can help the animal by identifying these indigestible compounds and feeding a suitable enzyme. These enzymes come from microorganisms that are carefully selected for the task and grown under controlled conditions (Wallis, 1996).

The biggest single expense in any system of poultry production is feed accounting for up to 70% of total production cost per bird. Poultry naturally produces enzymes to aid the digestion of feed nutrients. However, they do not have enzyme to break down fiber completely and need exogenous enzymes in feed to aid digestion. Plants contain some compounds that either the animal cannot digest, or which hinder its digestive system, often because the animal cannot produce the necessary enzyme to degrade them. Nutritionists can help the animal by identifying these indigestible compounds and feeding suitable enzyme. These enzymes come from microorganisms that are carefully selected for the task and grown under controlled conditions. (Creswell, 1994)

Anti-nutritional factors are problematic for normal feed digestion, results in low meat and egg production causes low feed efficiency and digestive upsets. Feed enzymes work to make the nutrient (starch, protein, amino acids and minerals, etc.) available from the feed ingredients. Feed enzymes also help to reduce the negative impact of animal production over environment by reducing the animal waste production. These Enzymes are proteins that are ultimately digested or excreted by the animal, leaving no residues in meat or eggs (Greiner and Konietzny, 2006).

The poultry industry readily accepts enzymes as a standard dietary component, especially in wheat and barley-based rations. But still many questions are partially answered. For example, how do enzymes work? Do growth rates reflect differences in the potency of different enzyme preparations? What is the link between gut viscosity, enzyme action and growth rates? and are enzymes necessary in all poultry rations? (Annison & Choct,1991).

2. Enzyme Supplementation in Poultry Ration

2.1. Enzyme
Enzymes are biological catalyst composed of amino acids with vitamins and minerals. They bring about biochemical reactions without themselves undergoing any change. They are involved in all anabolic and catabolic pathways of digestion and metabolism. Enzymes tend to be very specific catalysts that act on one or, at most, a limited group of compounds known as substrates. Enzymes are not living organisms and are not concerned about viability or cross infection. They are stable at 80-85 degree centigrade for short time. The benefits of using enzymes in poultry diets include not only enhanced bird performance and feed conversion but also less environmental problems due to reduced output of excreta. In addition, enzymes are a very useful tool in the study of physiological and metabolic mechanisms (Panda et al 2011).

2.2. Enzymes in Poultry Nutrition: The use of enzymes in animal feed is of great importance. Consistent increase in the price of feed ingredients has been a major constraint in most of the developing countries. As a consequence, cheaper and non-conventional feed ingredients have to be used which contain higher percentage of Non-Starch Polysaccharides (soluble and insoluble/crude fibre) along with starch. Non Starch Polysaccharides (NSPs) are polymeric carbohydrates which differ in composition and structure from starch (Morgan et al., 1995) and possess chemical cross linking among them therefore, are not well digested by poultry. A part of these NSPs is water-soluble which is notorious for forming a gel like viscous consistency in the intestinal tract (Ward et.al,1995) thus by reducing gut performance.

Poultry do not produce enzymes for the hydrolysis of Non-Starch Polysaccharide present in the cell wall of the grains and they remain un-hydrolysed. This results in low feed efficiency. Research work has suggested that the negative effects of NSPs can be overcome by dietary modifications including supplementation of diets with suitable exogenous enzyme preparations (Creswell, 1994). Enzymes break down the NSPs, decreases intestinal viscosity and eventually improve the digestibility of nutrients by improving gut performance.

Stallen South Asia Pvt Ltd has developed XZYME, a multi-enzyme formulation designed to optimize poultry feed utilization comprehensively. This innovative product combines various enzymes strategically selected to address specific nutritional challenges in poultry diets.

a) Cellulase
Cellulase is an enzyme complex that breaks down cellulose, a polysaccharide found in the cell walls of plants. Cellulose is composed of long chains of glucose molecules linked together by β-1,4-glycosidic bonds, making it a tough and fibrous substance that many animals, including poultry, cannot digest on their own. Cellulase enzymes help in hydrolyzing these bonds, converting cellulose into simpler, more digestible sugars.
b) Xylanase
Xylanase is an enzyme that hydrolyzes xylan into xylose, a simpler sugar. Xylan is a type of hemicellulose, which, like cellulose, is a polysaccharide present in plant cell walls. Xylanase breaks the β-1,4-glycosidic bonds in xylan, making it easier for poultry to digest plant-based feed ingredients.
c) β-Glucanase
β-Glucanase is an enzyme that plays a significant role in poultry nutrition by breaking down β-glucans, which are complex polysaccharides found in the cell walls of cereals such as barley, oats, and wheat. β-glucans are glucose polymers linked primarily by β-1,3 and β-1,4 glycosidic bonds. These β-glucans can be problematic in poultry diets because they increase the viscosity of the intestinal contents, hindering nutrient absorption and overall digestion. Here’s an overview of β-glucanase and its benefits in poultry nutrition.
d) Phytase
Phytase is an enzyme that catalyzes the hydrolysis of phytic acid (myo-inositol hexakisphosphate), a form of phosphorus that is commonly found in plant seeds and grains. Phytic acid binds phosphorus in a form that is not readily available to poultry because they lack sufficient endogenous phytase activity to break down this compound.
Phytase hydrolyzes phytic acid through a stepwise removal of phosphate groups, resulting in the release of inorganic phosphorus and lower inositol phosphates. This process occurs primarily in the stomach and upper small intestine of poultry, where the pH conditions are favorable for phytase activity.
e) Alpha-Amylase
Amylase acts on the α-1,4-glycosidic bonds within the starch molecule. Alpha-amylase randomly cleaves these bonds along the starch chain, resulting in the production of smaller carbohydrate molecules like maltose, dextrins, and glucose. These simpler sugars are then readily absorbed in the small intestine and utilized for energy.
f) Pectinase
Pectinase is an enzyme that catalyzes the hydrolysis of pectin, a structural polysaccharide in the cell walls of plants, particularly in fruits and vegetables. Pectin consists of a complex set of polysaccharides rich in galacturonic acid. Pectinases include a group of enzymes such as polygalacturonase, pectin lyase, and pectinesterase that break down pectin into simpler molecules like galacturonic acid, arabinose, and methanol which can be more readily absorbed by the poultry’s digestive system.
g) Protease
Protease is a type of enzyme that catalyzes the hydrolysis of peptide bonds within proteins, converting them into smaller peptides and free amino acids. These simpler molecules are more easily absorbed and utilized by the poultry for various physiological functions.
h) Lipase
Lipase enzymes work by hydrolyzing the ester bonds within triglycerides, breaking them down into free fatty acids and glycerol. This process primarily occurs in the small intestine, where lipase from the pancreas mixes with dietary fats, facilitating their breakdown and subsequent absorption by the intestinal cells.

3. Benefits of XZYME:

Benefits of using feed enzymes to poultry diets include; reduction in digesta viscosity, enhanced digestion and absorption of nutrients especially fat and protein, improved Apparent Metabolizable Energy (AME) value of the diet, increased feed intake, weight gain, and feed–gain ratio, reduced beak impaction and vent plugging, decreased size of gastrointestinal tract, altered population of microorganisms in gastrointestinal tract, reduced water intake, reduced water content of excreta, reduced production of ammonia from excreta, reduced output of excreta, including reduced N and P (Campbell et al. 1989).
a) Reduction in Digesta Viscosity: (Morgan et al,1995) found that that enzyme supplementation of wheat-based diets significantly reduced foregut digesta viscosity of birds. The reduction in foregut digesta viscosity was achieved primarily by reducing the molecular weight through hydrolysis of xylan backbone by endo-xylanase into smaller compounds and thus reduction in viscous effects of the feed because foregut digesta viscosity is directly proportional to the molecular weight of wheat arabinoxylans (Bedford and Classen, 1993).
b) Increase in Available Energy: One of the main reasons for supplementing wheat- and barley-based poultry diets with enzymes is to increase the available energy content of the diet. Increased availability of carbohydrates for energy utilization is associated with increased energy digestibility (Partridge and Wyatt ,1995). The AME of wheat has been extensively studied and found to have a considerable range i.e 9500–16640 kJ/kg (Mollah et al. 1983). Enzyme supplementation improves this range by enhancing carbohydrate digestibility, reducing gut viscosity, and improving fat utilization (Almirall et al. 1995).
c) Improvement in Nutrient Digestibility: Enzymes have been shown to improve performance and nutrient digestibility when added to poultry diets containing cereals, such as barley and wheat (Fengler et al. 1988).
d) Health improvement: Morgan and Bedford (1995) reported that coccidiosis problems could be prevented by using enzymes. Birds fed a wheat-based diet with and without glycanase supplementation showed vastly different responses to coccidiosis challenge. Growth was depressed by 52.5% in the control group but by only 30.5% in the enzyme group, which also had a much better lesion score. An increase in digesta passage rate and a reduction in excreta moisture are often noted when glycanases are added to poultry diets, which may be detrimental to the life cycle of the organism.
e) Impact on Environment: Enzymes have been approved for use in poultry feed because they are natural products of fermentation and therefore pose no threat to the animal or the consumer. Enzymes not only will enable livestock and poultry producers to economically use new feedstuffs, but will also prove to be environmentally friendly, as they reduce the pollution associated with animal production. As well as contributing to improved poultry production, feed enzymes can have a positive impact on the environment. In areas with intensive poultry production, the phosphorus output is often very high, resulting in environmental problems such as eutrophication.
This happens because most of the phosphorus contained in typical feedstuffs exists as the plant storage form phytate, which is indigestible for poultry. The phytase enzyme frees the phosphorus in feedstuffs and also achieves the release of other minerals (e.g. Ca, Mg), as well as proteins and amino acids bound to phytate. Thus, by releasing bound phosphorus in feed ingredients, phytase reduces the quantity of inorganic phosphorus needed in diets, makes more phosphorus available for the bird, and decreases the amount excreted into the environment.

Conclusion:
XZYME represents a significant advancement in poultry nutrition, offering a tailored solution to maximize feed efficiency and optimize poultry health. With its comprehensive enzyme blend and proven effectiveness, XZYME supports sustainable and profitable poultry production practices.

References:
Almirall, M., M. Francesch, A. M. Perez-Venderell, J. Brufau, and E. Esteve-Garcia. (1995). The differences in intestinal viscosity produced by barley and ß-glucanase alter digesta enzyme activities and ileal nutrient digestibilities more in broiler chicks than in cocks. Journal of Nutrition 125: 947–955.

Annison, G. and M. Choct. (1991). Anti-nutritive activities of cereal non-starch polysaccharides in broiler diets and strategies for minimizing their effects. World’s Poultry Science Journal 47: 232–242.

Bedford, M.R. and H. L. Classen. (1993). An in-vitro assay for prediction of broiler intestinal viscosity and growth when fed rye-based diets in the presence of exogenous enzymes. Poultry Science 72: 137-143.

Campbell, G.L., B. G. Rossnagel., H. L. Classen and P. A. Thacker. (1989). Genotypic and environmental differences in extract viscosity of barley and their relationship to its nutritive value for broiler chickens. Animal Feed Science and Technology 226: 221–230.

Creswell, D.C. (1994). Upgrading the nutritional value of grains with the use of enzymes. Technical bulletin, American Soybean Association, 341 Orchard Road No.11-03 Liat Towers, Singapore.
Fengler, A.I. and R. R. Marquardt. (1988). Water-soluble pentosans from rye. II. Effects on the rate of dialysis and on the retention of nutrients by the chick. Cereal Chemistry 65: 298–302.

Greiner, R., Konietzny, U., 2006. Phytase for food applications. Food Technol. Biotechnol., 44(2): 125-140.

Mollah, Y., Bryden, W.L., Wallis, I.R., D. Balnave and E. F. Annison. (1983). Studies on low metabolisable energy wheats for poultry using conventional and rapid assay procedures and the effects of processing. British Poultry Science 24: 81–89.

Morgan, A.J. and M. R. Bedford. (1995). Advances in the development and application of feed enzymes. Australian Poultry Science Symposium 7: 109–115.

Panda A.K., S. V. Rama Rao, M. V. L. N. Raju, M. R. Reddy and N. K. Praharaj. 2011. The Role of Feed Enzymes in Poultry Nutrition.

Partridge, G. and C. Wyatt (1995). More flexibility with new generation of enzymes. World Poultry 11(4), 17–21.

Wallis, I. (1996). Enzymes in poultry Nutrition. Technical Note, SAC.West Mains road, Edinburgh.

Ward, N.E. (1995). With dietary modifications, wheat can be used for poultry. Feedstuffs 7 Aug, 14-16.

Dr.Partha P. Biswas
M.Sc.,Ph.D.,F.Z.S.,F.Z.S.I.
Former Asso. Professor & H.O.D.,
Dept. of Zoology, R.K.Mission V.C.College,
Kolkata ,W.Bengal.
Senior Consultant, Aqua-Vet inputs,
Fin-O-Wing Formulations, Kolkata-700084

The chicken industry is becoming more vulnerable to environmental shifts, particularly high temperatures. Open-sided poultry species are susceptible to heat stress, negatively impacting growth and productivity. Factors determining heat stress include temperature radiation, humidity, metabolic rate, age, and duration. Modern commercial broilers are more sensitive to heat stress, making understanding and controlling environmental conditions crucial for poultry production and health. High temperatures in birds reduce antioxidant capacity, requiring food handling and expensive cooling. Understanding and controlling environmental conditions is crucial for poultry production and health.

Thermoregulatory Device in Chicken
Unlike mammals, birds do not have sweat glands, but they have developed a number of behavioral adaptations to cope with heat, including increased breathing rate, panting and raised wings. Commercial poultry prioritize high production, making broilers more sensitive to environmental stresses, and affecting meat quality and immune problems. Under conditions of heat stress, metabolic heat increases, and the animal succumbs to hyperthermia. In summary, it can be concluded that high ambient temperature outside the thermoneutral region during the production phase has a bad effect on meat production, meat quality and causes serious immune problems in broilers.

Heat Shock Proteins of Poultry Birds During Heat Stress
Heat shock proteins (HSPs) are stress proteins found in all living organisms that are activated by high environmental temperatures to protect cells from stressors such as heat. The 70 kDa heat shock proteins (HSP70) are a family of proteins known for their potential role in thermotolerance and widely regarded as cellular thermometers. Over expression of HSP70 has been observed under oxidative stress, leading to mitochondrial reactive oxygen species scavenging and pulmonary endothelial protection against bacterial toxins. They keep cells in order by synthesizing other proteins, attract immune cells and participate in protein assembly and degradation. Higher HSP expression is associated with better heat tolerance and is produced by all living organisms in high temperature environments.

Effects of Heat Stress in Poultry Birds
Reduced voluntary feed intake which affects the functionality of the entire digestive system High environmental temperatures activate the hypothalamus–pituitary axis, brain-gut axis and elevate plasma corticosterone concentrations, affecting the digestive system’s functionality.

This leads to changes in motility, flux patterns, secretory activity, content viscosity and pH Generation of ROS (reactive oxygen species) and the efficacy of the antioxidant defense system deteriorate. Overproduction of ROS in mitochondria can damage proteins, lipids, and DNA Heat stress can impair the feeding process, nutrient absorption and utilization, although water intake increases rapidly Upregulation of adipokines secretion (leptin and adiponectin) and the expression of their receptors can negatively regulate feed intake and calorie consumption thus resulting in decreased metabolic heat production The decline in trypsin, chymotrypsin and amylase (intestinal secretion) due to reduced feed intake often results in impairment of digestive functionality, nutrient digestibility Hypoperfusion and an increase in blood flow to the skin surface occur as an adaptive response of the circulatory system to stabilize blood pressure and promote heat loss It is known that heat challenge has an immune-suppressive effect.

Use of Dietary Phytochemicals to Reduce Heat Stress
Experimental studies on poultry birds suggest phytochemical ingestion may reduce heat stress effects. These phytochemicals can directly or indirectly influence genes and metabolic pathways, with stress reduction linked to antioxidant qualities.

Fig.3: The chicken’s response to being overheated. Chickens raised in high temperatures produce more reactive oxygen species and show signs of immunological inflammation in addition to consuming less food.

Mitigating Heat Stress Using Epigallocatechin-3-Gallate (EGCG), A Secondary Metabolite in Green Tea

Green tea’s most prevalent catechin, EGCG, is thought to be its most bioactive ingredient and possesses potent antioxidant properties. The primary cause of heat stress-induced oxidative stress in poultry is damage to tissues and cells, which is mostly manifested in an increase in MDA (malondialdehyde) concentration in such tissues and cells. It has been demonstrated that adding the polyphenol EGCG to broilers housed in thermoneutral environments may increase their antioxidant capacity. Acutely heat-stressed broilers may have greater antioxidant capacity and less oxidative damage in their muscles because EGCG may activate the Nrf2 signaling pathway.

Reducing Heat Stress in Broiler Chickens With Additional Ginger (Zingiber Officinale) and Onion (Allium Cepa)

Onion and its derivatives including saponins, aglycones, quercetin, cepaenes, flavonoids, organosulfurs, and phenolic compounds showed various pharmacological properties and therapeutic effects.When broilers are heat stressed, the combination of onion and ginger supplements increases the nutrition of the groups more than no supplementation.

According to research results, growth performance, carcass quality, antioxidant levels and immune system response of broilers are improved when fed 10 g of ginger and and 2.5 g of onion during heat stress. Ginger contains substances with powerful antibacterial and antioxidant properties, including chagaol, ginger diol and ginger diol. Ginger (2%) added to broilers suffering from heat stress significantly improved blood biochemical parameters and growth indicators compared to the control group.

Seeds of Black Cumin (Nigella Sativa) improve Bird’s Ability to Live in Heat-stressed Conditions

Black cumin seeds have been shown to have pharmacological and antibacterial properties and also contain drug-like compounds. The volatile oil (0.4-0.45%) contains saturated fatty acids, which include: nigellone, which is the only component of the carbonyl fraction. oil, thymoquinone (TQ), thymohydroquinone (THQ), dithymoquinone, thymol, carvacrol, α and β-pinene, d-limonene, d-citronellol, carvacrol, t-anethole, 4-terpineol and longifolin etc. Thymoquinone improves hatchability, pos-thatching performance and antioxidant activity of thermally stressed broiler embryos. Black cumin extract has been shown in trials to reduce serum MDA levels and protect against oxidative stress.

Hot Red Pepper (HRP) Reduces Heat Exhaustion in Birds

Ascorbic acid, or vitamin C, is abundant in capsaicin, a terpenoid found in HRP that helps prevent heat exhaustion in birds. Carotenoids, which are rich in vitamins E, C, and provitamin A (beta carotene), are known to have powerful antioxidant qualities that help prevent the damaging effects of free radicals and, in certain situations, oxidative stress, which can lead to cell death in broilers. Furthermore, it has been found that adding capsaicin, an active ingredient in red pepper that is present in grill feed at a dose of 50 mg/kg, can lessen the harmful effects of heat stress.

Moringa (Moringa Oleifera)helps to Survive Birds Under Heat Stress

Moringa leaves contain high levels of total polyphenols (260mg/100g), b-carotene (34mg/100g), kaempferol (34mg/100g), quercetin (100mg/100g), as well as a total antioxidant capacity of 260mg/100g. Kaempferol and quercetin are the flavonoids present in moringa leaves and possess strong antioxidants. It has been found that 0.3% incorporation of M. oleifera leaf meal improves the performance and physiological parameters of broilers and also helped the birds survive under heat stress.

THYME (THYMUS VULGAIS) Protects Chicks Against Heat Stress

The two most important bioactive compounds in this plant are carvacrol and thymol, which may be the primary source of thyme’s pharmacological actions. Thus research has identified linalool, thymol, carvacrol, gamma-terpineol, and geraniol as the primary components of thyme. Dietary thyme essential oil (150–200 mg/kg) is more effective at shielding chicks from the harmful effects of heat stress while also enhancing immunological function and development performance. One material that may be able to improve growth in broilers located in hot climates is thyme oil.

Coriander (Coriandrum Sativum) Seed in Ameliorating the Impact of Thermal Challenges

According to research, broilers under heat stress that are fed 2% coriander seed have higher feed intake, weight gain, reduced panting, and higher levels of corticosterone. The broilers’ poor intestinal absorptive capacity and shape may be connected to the rise in corticosterone levels during stress. Furthermore, according to a different study, adding 2% coriander to the diet helps broiler birds by lessening the effects of heat shock. The supplement, according to the author, benefitted broilers that were experiencing heat stress and enhanced their blood parameters, immunity, and overall performance.

Cinnamon (Cinnamomum Zeylanicum) Powder as Antioxidant in Thermally Challenged Birds

The common herbal plant, cinnamon contains different active phenolic compounds, which include flavones, catechin, isoflavones, flavonoids and other phenolics. The main bioactive constituent of cinnamon is cinnamaldehyde. The phenolic components function as antioxidants and can effectively scavenge ROS. Cinnamon supplements help in homeostasis due to the reduced pH caused by heat stress. It has also been reported that an increase in the activity of CAT, total antioxidant capacity and SOD and a decrease in the MDA when birds were placed in a thermally challenged environment during their finishing phase.

Turmeric (Curcuma Longa) for Heat-stressed Broilers

The yellowish pigments of turmeric, namely demethoxycurcumin, curcumin, and bisdemethoxycurcumin, are commonly referred to as curcumoids. Curcuminoids are an antioxidative compound found in turmeric. Researchers have shown the effects of turmeric powder supplement at 0.3 and 0.6 g/kg when administered to birds under heat stress. The superoxide radicals are neutralized, and there is an increase in the activity of SOD and CAT (ROS-removing enzymes or antioxidant enzymes ) and a decrease in MDA in broilers. The increased level in MDA indicates oxidative damage in liver of heat stressed broilers.

Conclusion
Heat stress can hurt poultry birds by making them grow slower, weakening their immune system, causing intestinal inflammation, and causing other health problems. It can also trigger oxidative process. But using natural substances called phytogenic compounds can help chickens who are raised in hot conditions.But more research is needed to understand the molecular changes made by medicinal herbs and the interactions between their active components, gut microbiota, and gut barriers. By using these approaches, we can improve chicken welfare and make poultry production more sustainable and efficient.

Spirulina Algae for Chickens – Nutritional Benefits and Commercial Potential

Dr.Partha P. Biswas M.Sc.,Ph.D.,F.Z.S.,F.Z.S.I.
Former Asso. Professor & H.O.D., Dept. of Zoology, R.K.Mission V.C.College, Kolkata ,W.Bengal.
Senior Consultant, Aqua-Vet inputs, Fin-O-Wing Formulations, Kolkata-700084

Natural ingredients are becoming more popular in chicken feed as a substitute for artificial colouring, antibiotics, and other chemicals that compromise human health and safety. One of the best natural feed additions for animals and poultry feed that improves nutritional content is spirulina, a microscopic alga. All of the necessary vitamins, minerals, and amino acids are present in spirulina. Moreover, it contains a wealth of fatty acids and carotenoids, particularly γ-Linolenic acid (GLA), which has been linked to positive health effects. But what sets spirulina apart as a novel animal feed is its high protein concentration (55 to 65%). For improved growth and decreased mortality, animal meals are supplemented with spirulina powder. Furthermore, these microalgae have been observed to have a high nutrient digestibility and an amino acid pattern that may be on par with or better than that of other vegetable diets and feeds. Aside from these, spirulina also includes colours (including β-carotene and zeaxanthin), phycobilin proteins (such phycocyanin, which is exclusive to cyanobacteria), vitamins, and macro and micro mineral components. These substances function as immunity boosters and colourants or disclose possible biological qualities as antibacterial, antioxidant, anti-cancer, and anti-inflammatory effects.

Spirulina is a type of blue green algae
These days, taxonomists, at least, agree that all Spirulina grown for commercial purposes belongs in the genus Arthrospira. Since this material is currently so well-known by this name, it appears inevitable that it will continue to be used; however, it should be written as Spirulina or spirulina, without the italics.

Spirulina is a microscopic algae, also known as blue-green algae. It belongs to the Cyanophyceae family. They feed themselves  through photosynthesis like plants, but their cells do not have a cellulose membrane like bacteria (which explains their very high digestibility, about 83%). The two most commonly used species are Spirulina platensis and Spirulina maxima. In India, Spirulina fusiformis is also considered a parent plant.

The name spirulina is derived from the Latin word meaning spiral or spiral. It is most often found in sea and brackish water. The blue- green color of the organism is due to the presence of several photosynthetic pigments such as chlorophyll, carotenoids, phycocyanin and phycoerythrin. Phycocyanin is responsible for the blue color of the body. According to the World Health Organization (WHO), spirulina is an interesting food rich in iron and protein and declared it as the best food of the future.

Fig.1 Scanning electron micrograph of morphology of Spirulina (Arthrospira)

Fig.2 Arthrospira platensis

Health benefits of Spirulina

1. Dietary supplementation of Spirulina can beneficially affect gut microbial population.
2. It affects serum biochemical parameters, and growth performance of chicken.
3.It contains polyphenolic contents having antibacterial effects.
4.It also has considerable quantities of unique natural antioxidants including polyphenols, carotenoids, and phycocyanin.
5. In addition to acting directly on the bacteria by weakening and increasing the permeability of the bacterial cell walls, which ultimately causes cytoplasmic content leakage. Spirulina extracts also inhibit bacterial motility, invasion, biofilm formation, and quorum sensing.
6. Spirulina has demonstrated antiviral properties against a number of common animal viruses, and it is possible that these properties could also be beneficial against viruses that infect birds. Spirulan, an internal polysaccharide of spirulina that is rich in calcium, may have an antiviral effect by preventing the entry of various viruses into host cells, increasing nitric oxide production in macrophages, and inducing the release of cytokines.
7. When added to chicken feed, it has immune modulatory effects that may increase resistance to disease and enhance survival and growth rates, especially in stressful situations.
8. The high nutrient digestibility of these microalgae is superior to or comparable with that of other vegetable diets and feeds.
9. Soybean meal in particular can be partially replaced by spirulina in place of more conventional protein sources.
10. In addition to the numerous Omega-3 and -6 polyunsaturated fatty acids that make up 25% and 60% of the total fatty acids in spirulina, other polyunsaturated fatty acids that are present in spirulina include oleic acid, linoleic acid, gamma-linolenic acid, docosahexaenoic acid (DHA), sulfolipids, and glycolipids.
11. Carotenoids, or pigments containing chlorophyll and β-carotene, are also present in spirulina (4000 mg/kg).
12. Spirulina also contains phytobiliproteins, vitamins, and macro- and micromineral elements such as calcium, iron, magnesium, manganese, potassium, zinc, and selenium. 13.In addition, pro-vitamin A, vitamin E, vitamin K, various B vitamins, polysaccharides, and antioxidants are all significant components of spirulina.
14.Hens and cocks showed significantly improved FCR when fed the basic diet supplemented with 2 or 3 g spirulina/kg diet during the laying period from 29 to 40 weeks of age.
15. A higher zinc content in spirulina such as this could be the cause of the improvement in cellular immunity seen in response to dietary supplementation of spirulina.
Tropical Climate in Tamil Nadu, South India, is perfect for Spirulina Cultivation

The warm tropical climate of Tamil Nadu is perfect for spirulina cultivation. In fact, certain varieties of spirulina grow naturally there. The ideal temperature range for growing spirulina in Tamil Nadu is between 25°C and 35°C. The best places to grow spirulina are also the ones that receive the most sunlight in the growing season. Spirulina is cultivated in large plastic or cement water tanks. The standard container size for spirulina is 10 x 5 x 1.5 feet, but containers can be of any size. It should be able to effectively pump 1000 liters of water, because this is the amount needed to fill to a height of 2-3 meters. After 3–4 weeks, or when the crop has reached a sufficient density, the crop can be harvested. When the spirulina is mature, it is pumped out of the pond to a collection point where the algae are filtered through stainless steel screens. The strained spirulina algae paste is then applied and washed three times with drinking water before it goes into a drying vessel, which turns it into a powder.Spirulina Hub, a Hyderabad-based enterprise, produces flakes, capsules, tablets, and powder for use in a range of dietary supplements.

Fig.4.Ladies involved in the spirulina industry

Morphology of Spirulina
Spirulina consisted of multicellular, filamentous, unbranched trichomes. The filaments were referred to as ‘trichomes’. The trichomes have a length of 50–500 μm and a width of 3–4 μm.The cells were cylindrical and the spiral was loose. There were gas filled vacuoles within the cells and the filaments had a helical shape. Multiplication occurs only by fragmentation of a trichome.

Fig.5. A Hyderabad based company is providing organic Spirulina powder in four different forms (powder, liquid, tablet & capsules, and flakes forms) to meet the needs of consumers for immediate use. (Permission taken for using this image for illustration purposes.)

Increases Immunological Functions in Chickens
In poultry, some recent studies have shown that feeding SP is responsible for improvement of immune functions, subsequently increased disease resistance, improved survival and growth rates Spirulina supplementation at 10,000 ppm ( = 1% ) level increased candidate NK-cell activity by two-fold over the controls. This may enhance disease resistance potential in chickens. Research conducted supplementation of the heat-exposed broilers diet with Spirulina and found enhanced humoral immunity response. Improvement in cellular immunity observed in response to dietary supplementation of Sprirulina might be attributed to higher Zn concentration in spirulina.

Better Serum Biochemistry by Spirulina Supplementation
Addition of up to 6 g/kg spirulina to the normal diet of broilers can improve the hematological and serum biochemistry of broiler chickens. Spirulina supplementation reduced serum urea and creatinine levels, suggesting that only microalgae promote more efficient nitrogen utilization. , which promotes a better balance between the body’s protein synthesis and the body’s protein breakdown.

Powerful Antioxidant Activity in Spirulina
Antioxidants counteract free radicals, preventing cell damage. ROS (reactive oxygen species), especially H2O2, disrupt the physiological equilibrium in tissues by breaking down biological components such proteins, lipids, and nucleic acids. SOD (superoxide dismutases) catalyses the dismutation(a type of redox process involving simultaneous reduction and oxidation) of hydrogen peroxide and molecular oxygen. SODs are the first line of defence against damage caused by reactive oxygen species (ROS).

These protect tissues from oxidative damage by converting superoxide radicals (O2-) into molecular O2 and H2O2.The primary enzyme in cells that scavenges hydrogen peroxidase and transforms it into water is glutathione peroxidase (GPx). SOD and GPx have the ability to directly offset oxidative stress and shield cells from DNA damage. Heat stress causes lipid peroxidation in cell membranes and increases corticosterone release, both of which promote oxidative tissue damage.Increased levels of MDA (mitochondrial malondialdehyde) and decreased activities of serum SOD and GPx in broilers exposed to high temperatures result in an imbalance in the oxidants/ antioxidants system and oxidative stress. Therefore, organic substances that counteract free radicals may help to rebalance the ratio of antioxidants to oxidants, promoting growth and better health. C-phycocyanin, a strong antioxidant, is one of the main components of Spirulina platensis. As a result, when compared to other heat-exposed birds in the current study, broiler chickens exposed to heat and fed 2% spirulina in their diet showed significantly lower MDA levels and higher SOD and GPx activities.

Feeding Spirulina & Chicken Meat Colour
One of the most crucial aspects that consumers consider when assessing fresh meat products is the color of the meat. Customers’ decisions about meat are largely influenced by the color and flavor of the meat. The color of chicken meat can be controlled with dietary spirulina, particularly in the range where the fillets made from feeding spirulina do not fall entirely into the dark or light meat categories. The high levels of carotenoids in the microalgae are probably what caused the darker, redder, and more yellow coloration of the breast filets fed on spirulina. According to some research findings, the common yellow pigment associated with the buildup of zeaxanthin in meat may correspond with the increase in yellowness associated with dietary spirulina content. Therefore, dietary spirulina is a powerful tool for adjusting the color of chicken meat. The addition of dietary spirulina at 1% of the total ration one week prior to slaughter has been found to produce the most consumer-preferred levels of muscle tissue pigmentation in broiler meat.

Spirulina Improves Chicken Meat Quality
The taste of the samples fed on spirulina was less metallic, and compared to the control group, the samples from the two alternate feed groups were softer and more tender. According to one study, adding S. platensis to broiler chicken diets can improve performance metrics, fatty and amino acid profiles, antioxidant status, and meat quality. In a similar vein, other researchers found that adding 15% of spirulina to broiler diets produced good-quality breast and thigh meat from chickens with higher levels of saturated fat, total carotenoids, and yellowness. As previously mentioned, feeding broilers spirulina, particularly at 1% and 2%, dramatically decreased the serum levels of total lipid, triglycerides, and cholesterol when compared to the control group.
Following spirulina supplementation, the fatty acid profile of the thigh meat of broiler chickens has been improved, particularly for eicosapentaenoic and docosahexaenoic acids.

Spirulina & Superior Egg Quality
In order to maximize egg production and maintaining flock health, feeding practices for laying hens are crucial. Particular focus is placed on the type, quantity, and caliber of protein provided in feeds. Egg quality is improved when spirulina is fed to the hens. The profitability of chicken production and consumer satisfaction are both impacted by the quality of the eggs produced. Egg weight, egg mass, and laying rate are all increased when 0.1%, 0.15%, and 0.2% spirulina is added to the diet, according to research. A useful natural feed supplement, spirulina at a concentration of 0.3% enhances the laying ability, egg quality, and hepatoprotective activity of hens. Feeding with spirulina considerably raises the average weight, color, and strength of the eggshell. A diet containing 2.0%-2.5% of spirulina significantly increases the egg yolk colour.

Conclusion
Animal nutritionists are paying close attention to alternative protein sources such as algae meals in order to substitute soybean meal (SBM). Spirulina microalgae meal appears to be a very viable option for SBM in broiler diets, at least in part. Spirulina has already been studied in relation to feeding additives for many of the most commonly farmed animal species. These trials’ outcomes have demonstrated increased output, better health, and higher-quality products. Many of the findings, nevertheless, run counter to one another. As a result, more spirulina research is required. In the near future, research on spirulina’s active components and associated biological pathways will contribute to our understanding of the plant’s potential, application, and implications for sustainable animal production.

AIPBA Chairman Shri Bahadur Ali, in a representation to Ministry of Fisheries, Animal Husbandry and Dairying, said ethanol makers’ growing thirst for maize has also pushed prices skyward, posing a major challenge for Indian poultry farmers. The All-India Poultry Breeders Association (AIPBA) demanded that the government permit duty-free import of maize to meet the requirement of the poultry industry amid rise in the grain consumption in ethanol production and insufficient domestic output.

With maize prices hovering around Rs 25-26 per kg across India, poultry farmers are grappling with unsustainable costs,” he said and cautioned that the burden is expected to intensify in the future, which may adversely impact the poultry industry. Against this backdrop, the association stated that there are two options before the government to address the rising demand for maize in both livestock feed and other industries. One option is importing maize, and the other is increasing domestic production.

“However, significant short-term increase in domestic output is deemed improbable. Therefore, importing maize from other countries emerges as the most viable solution to meet the immediate demand,” it stated in its representation. The current basic import duty on maize is 50 per cent. Citing concerns over the rising maize consumption in ethanol production, the association pointed out that India’s 34.60 million tonne annual maize production is insufficient to meet the requirements of the poultry industry as well as the nation’s food security.

As per estimates of the Indian Institute of Millets Research, the poultry and livestock industry consume more than 60 per cent of the country’s maize production, it said. In this context, the government’s ambitious plan to generate half of the ethanol from maize by 2025-26 “may have some serious implications for sectors like poultry and livestock.” The association said diverting such a significant chunk of current maize production could impact their access to essential feedstock, creating a severe demand-supply gap in the coming years. Also, maize production growth over the decade has been at 4.5 per cent, while the poultry industry has experienced a growth of 8-9 per cent. “This disparity highlights the anticipated maize shortage for the poultry industry, particularly in the wake of the government’s plan to promote maize for ethanol in a big way,” it observed. India is the sixth largest producer of maize in the world and its production in India is second only to wheat and rice.

To sustain growth in the poultry sector, the government must ensure supplies of feed ingredients at reasonable prices which should be ensured through liberalizing imports and augmenting production.

Livestock rearing is one of the most important economic activities in the rural areas contributing significantly to the economy. Livestock sector, although half the size of crops, plays a crucial role in driving the agricultural gross value added (GVA) growth. This sector is contributing to the economy in a big way considering the higher rate of growth of the sector in comparison to the agriculture sector.

Presently, the GVA of the livestock sector has recorded an annual growth rate of around 6% at constant prices. The growth of the sector is more than the crop sector growth rate which was 1.65% annually. Its contribution to the Indian agriculture and economy is increasing steadily with a share of 30.47% in agriculture and allied sector GVA and 4.75% in the country’s total GVA.

According to basic animal husbandry statistics, 2023, out of the total meat production of 9.77 million ton (MT) in 2022-23, the share of poultry meat was 4.99 MT, contributing 51% of total output. The growth of poultry meat production has increased by 4.52% over previous year. According to the Food and Agriculture Organisation, India ranks 8th in the world in terms of meat production. The poultry sector in India is valued at more than $28 billion in 2021-22, according to the Confederation of Indian Industry (CII)’s vision document – 2047 for Indian poultry sector released recently. Over the years, the poultry sector in the country has witnessed a remarkable growth, with chicken meat growing at an annual growth rate of 8% in the last 15 years – 2006-2021-22, the report stated.

With rising disposable income and population, the demand for poultry products including chicken meat and eggs has been on rise. The sector has capitalized on this opportunity and expanded its production capability to meet the growing consumer demand. This significant transformation in the poultry sector has been attributed to the commercial poultry industry which accounts for 85% of the total poultry production and 15% is contributed by backyard poultry. The sector has witnessed a shift from the traditional backyard poultry models to a model production technique including integrated farming systems, contracting farming and value chain integration.

As the share of meat and egg eating population has increased by 6% during 2015 – 2021, the national family health survey -V, 2021, the demand for poultry and products is set to increase further. Currently, the per capita consumption of poultry products in the country (94 eggs per annum and chicken meat consumption is 4.2 kg per annum) is very low as compared to the Indian Council for Media Research (ICMR) recommended consumption level of 180 eggs and 10.8/kg poultry meat per person per annum. There is a need to bridge the gap between availability and requirements couples with large scale awareness campaigns.

For enhancing efficiency of the poultry sector, several measures are being undertaken to improve genetics and disease resistance breeds of poultry, disease prevention and surveillance, and supply of affordable feed which constitute 65% to 70% of the cost of production of meat remain a challenge. While stating that the domestic poultry industry is likely to grow at a steady pace of 8%-10% in 2023-24, consulting firm ICRA in March this year had stated that earnings of poultry companies are expected to be volatile owing to fluctuations in the raw material or feed costs, especially maize. ICRA has stated that due to rising worldwide demand for Indian maize as a result of the Russia-Ukraine conflict and increased exports from India, maize prices have grown significantly by 32% on a year-on-year (YoY) basis in 2022-23, resulting in increase in average feed price.

Poultry feed mostly consists of maize, Bajra and broken rice (60-65%), soybean meal (30-35%) and nutrients. The mandi prices of maize because of rising demand for industrial use is ruling much above the minimum support price (MSP) of Rs.1962/quintal announced by the government for 2022-23 and Rs.2090/quintal for 2023-24 kharif season. Stating that there has been increasing diversion of maize towards industrial use and ethanol production, “the current growth level of maize and soybean production in the country will be insufficient to meet the demand of the poultry industry.” As the government plans to promote use of maize for ethanol, the poultry industry can face challenges in getting maize for feed.

Several poultry and livestock industry associations including All India Poultry Breeders Association, The Compound Livestock Feed Manufacturers Association, Poultry Federation of India, Vets in Poultry, are now Pitching for the government to allow imports of GM maize and soybean because of ‘unprecedented increase’ in prices. Regional Poultry Associations have also urged the central government for reduction in import duty on maize and soybean to deal with feed supplies. The industry feared that the prices of maize would spike in the coming months as the diversion of these raw materials for ethanol production is expected to increase as the government has reduced allocation of sugarcane for biofuel production.

In August 2021, the government had relaxed import rules to allow the first shipment of 1.2 MT of genetically modified (GM) soymeal to support the domestic poultry industry after a record spike in feed prices. Poultry industry has requested that the Government should allow both import and cultivation of GM Soybeans and Maize to fulfill the requirement of these two major feed ingredients. The sustainable supplies of feed ingredients in coming years would be crucial for the growth of the poultry industry.

Dr. Sundus Gazal1, Dr. Sabahat Gazal2, Dr. Anvesha Bhan3 and Dr. Shalini Pandey4
1,2,3Division of Veterinary Microbiology and Immunology, SKUAST-Jammu
4Department of Veterinary Microbiology, RPS Veterinary College, Mahendragarh

Introduction:
Poultry farming is an integral part of the global food supply chain, meeting the demand for protein-rich meat and eggs. Achieving optimal growth, health, and productivity in poultry operations is essential for farmers to meet market demands and ensure profitability. A critical factor in achieving these goals lies in implementing effective feeding strategies. In this article, we will explore in-depth the various facets of poultry feeding that can lead to increased productivity, streamlined operations, and improved economic outcomes.

Nutrient Balance:
At the core of successful poultry feeding is providing a balanced and nutritionally rich diet as poultry convert feed into food products quickly, efficiently, and with relatively low environmental impact relative to other livestock. Their high rate of productivity results in relatively high nutrient needs. Poultry require a complex combination of nutrients including proteins, carbohydrates, fats, vitamins, and minerals for their growth and well-being. Feed ingredients for poultry diets are selected for the nutrients they can provide, the absence of anti-nutritional or toxic factors, their palatability or effect on voluntary feed intake, and their cost. Poultry require at least 38 nutrients in their diets in appropriate concentrations and balance. These nutritional requirements evolve as birds progress from chicks to mature individuals, necessitating careful adjustment of feed formulations at different growth stages depending on the requirements related to production (e.g., growth, feed efficiency, egg production), prevention of deficiency symptoms, and quality of poultry products.

Feed Formulation:
Feed formulation involves quantification of the amount of feed ingredients required to be combined to form a single uniform balanced diet for poultry which can supply all the nutritional requirements of the birds. Since feed accounts for 65-75% of total live production costs for most types of poultry throughout the world, a simple mistake in diet formulation can be extremely expensive for a poultry producer. It requires a thorough understanding of the nutrient requirements of the class of poultry (e.g., egg layers, meat chickens or breeders); feed ingredients in terms of nutrient composition and constraints in terms of nutrition and processing; and cost and availability of the ingredients. The quality of feed directly impacts the health and growth of poultry. Opting for high-quality ingredients ensures that the feed is easily digestible and minimizes wastage. In addition to energy and protein, the formulations should also contain supplements to provide minerals, vitamins and specific amino acids.

These supplements must be added to all diets as they provide essential nutrients necessary for health and performance. Modern feed formulations also contain a diverse range of non-nutritive additives, which may not be essential but have an important bearing on performance and health. A major factor to be considered in selecting these additives is their efficacy as they are used in only small quantities, which makes it particularly important that they are mixed carefully with the main ingredients so that they are evenly distributed. Beyond ingredient selection, the form of feed also matters. Pelleted or crumbled feeds have been shown to enhance feed efficiency, minimize waste, and improve nutrient absorption. There are several systems of feeding: free-choice or “cafeteria style” feeding of mash and grain, controlled feeding of mash and grain, feeding all mash, or other combinations of a complete feed. Each system should accommodate the specific needs of the flock, and be designed for flexibility, low maintenance, and reliability to keep installation and operating costs low. The choice of one of these feeding systems will depend mainly upon the size of the flock and the labour and equipment available.

Incorporating feed additives further supports digestive health and nutrient utilization. These additives are primarily included to improve the efficiency of the bird’s growth and/or laying capacity, prevent disease and improve feed utilisation. Common feed additives used in poultry diets include probiotics, prebiotics, antimicrobials, antioxidants, emulsifiers, binders, pH control agents, enzymes, flavour enhancers, artificial and nutritive sweeteners, colours, lubricants, etc. Modern intensive poultry production has achieved phenomenal gains in the efficient and economical production of high quality and safe chicken meat, eggs and poultry bioproducts by the use of properly balanced high quality feed and feed additives.

Feeding Programs:
Developing a structured feeding schedule is a cornerstone of effective poultry management. Birds of different ages have distinct nutritional requirements. Therefore, a well-designed feeding program must align with the specific age and purpose of the poultry – whether they are being raised for meat or egg production. Gradual transitions between feed formulations during growth phases prevent digestive disturbances and ensure a smooth progression from one growth stage to another.

Water Management:
Water is a critical, but often overlooked, nutrient. Uninterrupted access to clean and fresh water is a fundamental aspect of poultry health and growth. Adequate hydration plays a pivotal role in determining feed intake and metabolic processes. A consistent supply of clean water supports efficient nutrient absorption, aids in digestion, and contributes to the overall well-being of the birds. Effective water management complements the feeding strategy and maximizes its impact.

Feeding Space and Equipment:
Creating an environment that minimizes stress during feeding times is crucial. Sufficient feeding space is essential to prevent competition and aggression among birds, ensuring equitable access to feed. Without a good feed distribution and sufficient feeder space the smaller or less aggressive birds will not get their share of the available daily feed amount, and uniformity will suffer. Employing appropriate feeding equipment that minimizes wastage while facilitating easy access to feed enhances consumption efficiency and reduces unnecessary costs. A good feeder should be durable enough to withstand frequent cleaning; stable enough not to be knocked over; of the correct height and depth; bird proof (such that birds cannot get into it or roost in it); and equipped with a lip to prevent birds from spooning feed out onto the floor with their beaks. The height of the feed inside the feeder, which should never be more than one-third full, should be level with the back of the birds, to prevent them from scratching contaminated litter into the feeders and to limit feed wastage.
Feeders can be made of wood, sheet metal or bamboo, and are best suspended from the roof to keep rats out.

Natural Foraging and Enrichment:
Encouraging natural behaviors among poultry is vital for their welfare and productivity. Allowing outdoor access or enriching the indoor environment with opportunities for foraging, pecking, and exploration engages the birds both physically and mentally. Environmental enrichment strategies are used to help prevent boredom and improves the physical and mental wellbeing of the flock. It helps reduce bullying amongst flock members, improves mental and physical health, and decreases the likelihood of injuries. Enrichment strategies are aimed at increasing opportunities for the animals to engage in natural behaviours that they would normally do in the wild. The five different forms of enrichment include cognitive, foraging (food), social, sensory, and environmental. This engagement reduces stress levels and enhances overall health, translating into improved productivity.

Monitoring and Record Keeping: Record keeping involves taking notes about what happens on the farm and involves information with regards to feed, water, medicine, and other items used on the farm. It also includes documenting any problems or events that happen on the farm and includes regular assessment of the growth and well-being of the flock. Maintaining accurate records of key metrics such as feed consumption, weight gain, and mortality rates provides valuable insights into the success of the chosen approach. This data-driven approach empowers farmers to make informed adjustments and continuously refine their feeding strategy. Records tell a manager where the business/operation has been and the direction in which it is going. Records show the strength and weaknesses of the poultry operation. They provide useful insight to financial stability for the flock. If there are any shortcomings, records will show where adjustments can be made.

Health and Biosecurity:
A robust health management strategy is pivotal in ensuring optimal feed intake and growth. A poultry operation’s success or failure can be dramatically affected by biosecurity, which is the effective use of standard hygienic practices. Biosecurity comprises of the Structural biosecurity which includes all facets pertaining to facilities and equipment; and Operational biosecurity which refers to normal tasks carried out on a farm on a regular basis, such as staff entry, vehicle entry and disinfection, pest management, garbage disposal, etc. Stringent biosecurity measures help prevent the introduction and spread of diseases that can disrupt feed consumption and growth rates. Regular veterinary supervision is essential to monitor flock health, identify potential issues, and recommend appropriate interventions. A strong health foundation lays the groundwork for the effectiveness of the feeding strategy.

Adaptation and Innovation:
The field of poultry nutrition is dynamic, with new technologies and methodologies constantly emerging. Poultry farmers must stay informed about advancements and be willing to embrace innovative practices. Artificial intelligence is a powerful tool that could help poultry produces improve efficiency and address welfare and health challenges. This technology has many possible applications for poultry operations. Examples include machine learning, camera vision and acoustic monitoring to improve bird welfare and share data with veterinarians. Automation can be used to replace manual labor on poultry farms when it comes to repetitive tasks like checking bird welfare, removing welfare, vaccinations and managing litter. In addition to this, precision feeding, automated systems, and data analytics are examples of innovations that can fine-tune feed efficiency, reduce costs, and amplify overall productivity.

Economic Considerations:
Profitability is a central concern for poultry farmers. Feed is the major component of input cost, accounting for up to 70% of the total production cost. Effective feeding strategies strike a balance between input costs and output gains. Conducting comprehensive cost-benefit analyses empowers farmers to make informed decisions regarding feed formulations, feed conversion ratios (FCR), and other critical factors that directly influence the financial bottom line.

Conclusion:
Implementing a thoughtfully designed feeding strategy is the linchpin of successful poultry farming. By focusing on nutrient balance, feed formulation, feeding schedules, and other key elements, poultry farmers can elevate the productivity and profitability of their operations. Continuous monitoring, adaptability to new techniques, and unwavering commitment to bird health are instrumental in ensuring long-term success in the ever-evolving realm of poultry farming. Through these efforts, poultry farmers not only meet the global demand for food but also uphold the well-being of their flocks and the sustainability of their operations.