Introduction
The poultry industry has seen exponential growth over the last few decades, driven by the demand for high-quality protein sources such as chicken. However, the intensification of poultry production has also brought challenges, particularly in managing the health of broilers, which are reared under conditions that can predispose them to stress and diseases. Among these, gut health is a critical area of focus because it directly influences the overall health, performance, and productivity of the birds.

Traditionally, antibiotics have been used extensively to manage gut health issues and prevent diseases. However, the rise of antimicrobial resistance (AMR) and global consumer demand for antibiotic-free poultry has necessitated a shift toward non-antibiotic solutions. Phytomolecules, bioactive compounds derived from plants, have emerged as a promising alternative for maintaining gut health in broilers. This article delves into the significance of gut health in broilers, explores the role of phytomolecules and highlights their effectiveness as a sustainable solution in modern poultry operations.

Understanding Gut Health in Broilers
Gut health refers to the optimal functioning of the gastrointestinal (GI) tract, which is essential for nutrient absorption, immune response, and overall well-being of broilers. In poultry, the gut is not only responsible for digestion but also acts as a key barrier against pathogens, playing a critical role in the immune system. (Image 1)

(Image 1) Source: Guillermo Tellez-Isaias et al 2023, Engormix

A healthy gut consists of a balanced microbial population (microbiota), an intact intestinal barrier, and a well-regulated immune response. Any imbalance in these components can lead to gut dysfunction, manifesting as poor nutrient absorption, diarrhoea, increased susceptibility to infections, and reduced growth performance.

Common gut health challenges in broilers include:
1. Dysbiosis: An imbalance in the gut microbiota, often caused by stress, poor nutrition, or infections, can disrupt gut function.
2. Enteric diseases: Diseases like necrotic enteritis (caused by Clostridium perfringens) and coccidiosis (caused by Eimeria species) can severely damage the intestinal lining.
3. Leaky gut syndrome: Increased intestinal permeability can allow harmful substances to pass into the bloodstream, triggering inflammation and immune responses.
4. Poor nutrient absorption: Impaired gut function can reduce the efficiency of nutrient absorption, affecting growth rates and feed conversion ratios.

Source: Self Field observations

Maintaining optimal gut health is, therefore, essential to achieving high productivity, reducing mortality, and ensuring efficient feed utilization in broilers.

The Role of Phytomolecules in Gut Health
Phytomolecules are bioactive compounds derived from plants, including essential oils, alkaloids, flavonoids, tannins, and terpenes. These molecules possess a wide range of biological activities, such as antimicrobial, antioxidant, anti-inflammatory, and immunomodulatory properties, making them effective in maintaining and improving gut health.

Over the years, research has demonstrated the potential of phytomolecules to support gut health in poultry. Several studies have shown that these plant-derived compounds can modulate the gut microbiota, strengthen the intestinal barrier, and enhance immune responses, thus promoting better growth and health in broilers.

1. Antimicrobial Properties
One of the primary benefits of phytomolecules is their ability to exert antimicrobial effects. Many essential oils and plant extracts contain compounds like carvacrol, thymol, and eugenol, which have been found to inhibit the growth of pathogenic bacteria such as Escherichia coli, Salmonella, and Clostridium perfringens. These antimicrobial properties help maintain a balanced gut microbiota, reducing the risk of infections and dysbiosis. (Image 2)

A study by Burt (2004) demonstrated that essential oils containing carvacrol and thymol are effective in inhibiting the growth of Salmonella and Campylobacter in broilers. Similarly, Liu et al. (2012) found that phytogenic compounds such as oregano and thyme oils can significantly reduce the colonization of pathogenic bacteria in the poultry gut.

2. Antioxidant Effects
Oxidative stress is a common challenge in modern poultry production, especially under intensive farming conditions. Excessive oxidative stress can damage the intestinal lining, leading to inflammation and compromised gut integrity. Phytomolecules such as flavonoids and phenolic acids have strong antioxidant properties, which help neutralize free radicals and protect the intestinal cells from oxidative damage. (Image 3)

Flavonoids, such as quercetin and catechins, have been shown to enhance the activity of antioxidant enzymes, reduce inflammation, and promote gut integrity. In a study conducted by Rehman et al. (2020), supplementation with flavonoid-rich plant extracts improved the gut health of broilers by reducing oxidative stress and enhancing the intestinal barrier function.

(Image 3) Source: Yammine, Jina et al. Heliyon, Volume 8, Issue 12, e12472

3. Anti-inflammatory Action
Chronic inflammation in the gut can lead to poor nutrient absorption, tissue damage, and increased susceptibility to infections. Phytomolecules possess anti-inflammatory properties that can mitigate gut inflammation and support tissue repair. Compounds such as curcumin (found in turmeric) and gingerols (found in ginger) are well-known for their anti-inflammatory effects. A study by Khaleel et al. (2021) demonstrated that dietary supplementation with curcumin significantly reduced gut inflammation in broilers and improved their overall performance. Similarly, ginger extract has been found to decrease pro-inflammatory cytokines and enhance gut health in poultry.

4. Enhancing the Intestinal Barrier
The intestinal barrier is the first line of defence against harmful pathogens and toxins. Phytomolecules, particularly tannins and essential oils, can strengthen the intestinal lining by promoting the production of tight junction proteins that seal the spaces between intestinal cells. This helps reduce intestinal permeability (leaky gut) and prevents the translocation of harmful substances into the bloodstream. (Image 4)

In a study by Yang et al. (2015), tannin-rich plant extracts were found to enhance the expression of tight junction proteins in the intestinal mucosa of broilers, resulting in improved gut integrity and reduced incidence of leaky gut.

5. Modulating the Gut Microbiota
Phytomolecules have prebiotic effects that promote the growth of beneficial gut bacteria, such as Lactobacillus and Bifidobacterium, while inhibiting pathogenic bacteria. A balanced gut microbiota plays a crucial role in maintaining gut health by enhancing nutrient absorption, stimulating the immune system, and protecting against infections.

Research by Windisch et al. (2008) found that phytogenic feed additives, including essential oils and polyphenols, can modulate the gut microbiota by promoting beneficial bacteria and reducing pathogenic bacterial populations. This microbiota modulation helps maintain gut homeostasis, which is essential for optimal growth and performance in broilers.

Phytomolecules in Commercial Broiler Production
The use of phytomolecules as feed additives in broiler production is gaining popularity as a natural and effective alternative to antibiotics. Various commercial phytogenic products containing essential oils, plant extracts, and other bioactive compounds are now available for use in poultry diets.

Benefits of Phytomolecules Supplementation
1. Improved Growth Performance: Several studies have shown that phytomolecules supplementation can enhance growth rates, feed conversion ratios, and overall performance in broilers. For example, Yang et al. (2015) reported that broilers supplemented with a blend of essential oils and polyphenols exhibited higher weight gain and better feed efficiency.

2. Reduced Mortality and Morbidity: By promoting gut health and enhancing the immune system, phytomolecules help reduce the incidence of enteric diseases and lower mortality rates in broilers. A study by Ciftci et al. (2010) found that broilers fed with a diet containing thyme and rosemary essential oils had a lower incidence of necrotic enteritis and improved survival rates.

3. Enhanced Feed Efficiency: Phytomolecules improve nutrient absorption by maintaining gut integrity and supporting the activity of digestive enzymes. This leads to better feed efficiency and reduced feed costs, which are critical factors in commercial broiler production.

4. Sustainability and Consumer Acceptance: The use of phytogenic feed additives aligns with the growing consumer demand for antibiotic-free poultry products. As these additives are derived from natural sources, they are perceived as safe and environmentally friendly, contributing to the sustainability of poultry production.

Challenges and Considerations
While the benefits of phytomolecules in poultry production are well-documented, there are some challenges associated with their use.
These include:

– Variability in Efficacy: The efficacy of phytomolecules can vary depending on factors such as plant source, extraction method, dosage, and the overall diet composition. Standardization of phytogenic products is essential to ensure consistent results.

– Cost: Phytogenic feed additives can be more expensive than traditional antibiotics. However, the long-term benefits, including improved bird health and performance, can offset the higher initial costs.

– Regulatory Approval: Globally in some regions, the use of certain phytomolecules in animal feed may be subject to regulatory approval. Producers should ensure that the phytogenic products they use comply with local regulations.

Conclusion
Gut health is a cornerstone of successful broiler production, influencing not only the health and welfare of the birds but also their growth performance and profitability. As the poultry industry continues to shift toward antibiotic-free production systems, phytomolecules offer a natural and effective solution for maintaining gut health in broilers.
By leveraging the antimicrobial, antioxidant, anti-inflammatory, and microbiota-modulating properties of phytomolecules, poultry producers can improve gut integrity, reduce the incidence of enteric diseases, and enhance the overall performance of their birds. The multiple mechanisms through which phytomolecules support gut health, such as promoting beneficial microbial populations, protecting the intestinal barrier, and mitigating oxidative stress, make them a valuable tool in the pursuit of sustainable poultry production.

The growing body of research supporting the efficacy of phytomolecules in improving broiler gut health underscores their potential as a reliable alternative to antibiotics. Studies have consistently demonstrated that these plant-derived compounds can improve growth performance, reduce mortality, and enhance feed efficiency, all while aligning with consumer demands for natural, antibiotic-free products.

In conclusion, phytomolecules represent a promising, natural solution for enhancing gut health in broilers, offering benefits that extend beyond disease prevention to improving overall flock performance. As the poultry industry moves toward more sustainable and consumer-friendly practices, phytomolecules will likely play an increasingly important role in maintaining the health and productivity of broilers in antibiotic-free production systems.
The future of broiler production lies in sustainable practices that prioritize animal health and welfare without relying on antibiotics. Phytomolecules offer a natural and scientifically backed solution to the challenges of maintaining gut health in broilers, making them a critical component of the next generation of poultry feed additives.

References:
References are available on request.

Most grains used in feed are susceptible to mycotoxin contamination, causing severe economic losses all along feed value chains. As skyrocketing raw material prices force producers to include a higher proportion of economical cereal byproducts in the feed, the risks of mycotoxin contamination likely increase. In this article, we review why mycotoxins cause the damage they do – and how effective toxin-mitigating solutions prevent this damage.

Mycotoxin contamination of cereal byproducts requires solutions
Cereal byproducts may become more important feed ingredients as grain prices increase. But also from a sustainability point of view and considering population growth, using cereal byproducts in animal feed makes a lot of sense. Dried distiller’s grains with solubles (DDGS) are a good example of how byproducts from food processing industries can become high-quality animal feed.

Figure 1: Byproducts are a crucial protein source (data from FEFAC Feed & Food 2021 report)
Still, research on what happens to mycotoxins during food processing shows that mycotoxins are concentrated into fractions that are commonly used as animal feed
(cf. Pinotti et al., 2016.) To safeguard animal health and performance when feeding lower-quality cereals, it is essential to monitor mycotoxin risks through regular testing and to use toxin-mitigating solutions.

Problematic effects of mycotoxins on the intestinal epithelium
Most mycotoxins are absorbed in the proximal part of the gastrointestinal tract. This absorption can be high, as in the case of aflatoxins (ca. 90%), but also very limited, as in the case of fumonisins (< 1%); moreover, it depends on the species. Importantly, a significant portion of unabsorbed toxins remains within the lumen of the gastrointestinal tract.

Importantly, studies based on realistic mycotoxin challenges (e.g., Burel et al., 2013) show that the mycotoxin levels necessary to trigger damaging processes are lower than the levels reported as safe by EFSA, the Food Safety Agency of the European Union. The ultimate consequences range from diminished nutrient absorption to inflammatory responses and pathogenic disorders in the animal (Figure 2).

1. Alteration of the intestinal barrier ‘s morphology and functionality
Several studies indicate that mycotoxins such as aflatoxin B1, DON, fumonisin B1, ochratoxin A, and T2, can increase the permeability of the intestinal epithelium of poultry and swine (e.g. Pinton & Oswald, 2014). This is mostly a consequence of the inhibition of protein synthesis.

As a result, there is an increase in the passage of antigens into the bloodstream (e.g., bacteria, viruses, and toxins). This increases the animal’s susceptibility to infectious enteric diseases. Moreover, the damage that mycotoxins cause to the intestinal barrier entails that they are also being absorbed at a higher rate.

2. Impaired immune function in the intestine
The intestine is a very active immune site, where several immuno-regulatory mechanisms simultaneously defend the body from harmful agents. Immune cells are affected by mycotoxins through the initiation of apoptosis, the inhibition or stimulation of cytokines, and the induction of oxidative stress.

For poultry production, one of the most severe enteric problems of bacterial origin is necrotic enteritis, which is caused by Clostridium perfringens toxins. Any agent capable of disrupting the gastrointestinal epithelium – e.g. mycotoxins such as DON, T2, and ochratoxin – promotes the development of necrotic enteritis.

3. Alteration of the intestinal microflora
Recent studies on the effect of various mycotoxins on the intestinal microbiota show that DON and other trichothecenes favor the colonization of coliform bacteria in pigs. DON and ochratoxin A also induce a greater invasion of Salmonella and their translocation to the bloodstream and vital organs in birds and pigs – even at non-cytotoxic concentrations.

It is known that fumonisin B1 may induce changes in the balance of sphingolipids at the cellular level, including for gastrointestinal cells. This facilitates the adhesion of pathogenic bacteria, increases in their populations, and prolongs infections, as has been shown for the case of E. coli. The colonization of the intestine of food-producing animals by pathogenic strains of E. coli and Salmonella also poses a risk for human health.

4. Interaction with bacterial toxins
When mycotoxins induce changes in the intestinal microbiota, this can lead to an increase in the endotoxin concentration in the intestinal lumen. Endotoxins promote the release of several cytokines that induce an enhanced immune response, causing inflammation, thus reducing feed consumption and animal performance, damage to vital organs, sepsis, and death of the animals in some cases.

The synergy between mycotoxins and endotoxins can result in an overstimulation of the immune system. The interaction between endotoxins and estrogenic agents such as zearalenone, for example, generates chronic inflammation and autoimmune disorders because immune cells have estrogen receptors, which are stimulated by the mycotoxin.

Increased mycotoxin risks through byproducts? Invest in mitigation solutions.
To prevent the detrimental consequences of mycotoxins on animal health and performance, proactive solutions are needed that support the intestinal epithelium’s digestive and immune functionality and help maintain a balanced microbiome in the GIT. As the current market conditions will likely engender a long-term shift towards the inclusion of more cereal byproducts in animal diets, this becomes even more important.

Trial data shows that EW Nutrition’s toxin-mitigating solution SOLIS MAX provides effective protection against feedborne mycotoxins. The synergistic combination of ingredients in SOLIS MAX mycotoxins from damaging the animals’ gastrointestinal tract and entering the blood stream:

In-vitro study shows SOLIS MAX’ strong mitigation effects against wide range of mycotoxins
Animal feed is often contaminated with two or more mycotoxins, making it important for an anti-mycotoxin agent to be effective against a wide range of different mycotoxins. A dose response evaluation of SOLIS MAX was conducted a at an independent laboratory in Spain, for inclusion levels of 0.10%, 0.15%, and 0.20% (equivalent to 1 kg, 1.5 kb, and 2 kg per ton of feed). A phosphate buffer solution at pH 7 was prepared to simulate intestinal conditions in which a portion of the mycotoxins may be released from the binder (desorption).

Each mycotoxin was tested separately by adding a challenge to buffer solutions, incubating for one hour at 41°C, to establish the base line (see table). At the same time a solution with the toxin challenge and SOLIS MAX was prepared, incubated, and analyzed for the residual mycotoxin. All analyses were carried out by high performance liquid chromatography (HPLC) with standard detectors.

The results demonstrate that SOLIS MAX is a very effective solution against the most common mycotoxins found in raw materials and animal feed, showing clear dose-response effects.

Mycotoxin risk management for better animal feed
A healthy gastrointestinal tract is crucial to animals’ overall health: it ensures that nutrients are optimally absorbed, it provides effective protection against pathogens through its immune function, and it is key to maintaining a well-balanced microflora. Even at levels considered safe by the European Union, mycotoxins can compromise different intestinal functions, resulting in lower productivity and susceptibility to disease.

The globalized feed trade, which spreads mycotoxins beyond their geographical origin, climate change and raw material market pressures only escalates the problem. On top of rigorous testing, producers should mitigate unavoidable mycotoxin exposures through the use of solutions such as SOLIS MAX – for stronger animal health, welfare, and productivity.

References are available on request.

Author:
DEEP CHAND VASHISHTHA -M.Sc , MBA
NSM- Bioncia International Pvt Ltd

Stress comes in many forms and seems to affect the performance of birds. The term “stress” is used to describe the detrimental effect of variety of factors on the health and performance of poultry (Rosales, 1994) Or “Stress is the nonspecific response of the body to any demand”, whereas stressor can be defined as “an agent that produces stress at any time”. Therefore, stress represents the reaction of the animal organism (i.e., a biological response) to stimuli that disturb its normal physiological equilibrium or homeostasis (Selye, 1976). The commercial high yielding breeds are more susceptible to stress and diseases. Stress represents the reaction of the animal organism (i.e., a biological response) to stimuli that disturb its normal physiological equilibrium or homeostasis. The importance of animal responses to environmental challenges applies to all species. However, poultry seems to be particularly sensitive to temperature-associated environmental challenges, especially heat stress. Understanding and controlling environmental conditions is crucial to successful poultry production and welfare. Heat Stress not only causes suffering and death in the birds, but also results in reduced or lost production that adversely affects the profit from the enterprise.

Heat stress or any type of Stress have side effect on Vital organs heart, brain, kidneys, liver, and lungs.
Heat Stress adverse effects on liver
The liver is pivotal organ of metabolic activity, which performs essential cellular functions containing the balance of energy metabolism, biosynthesis of vitamins and minerals, and ammonia detoxification (Schliess et al., 2014). Elevated blood flow transfers from the hepato-splanchnic region to respiratory muscles and superficial body tissues to accelerate heat dissipation and decrease body temperature under heat stress, therefore, liver is more sensitive to heat stress (Hai et al., 2006; Crandall et al., 2008). It has been reported that heat stress caused liver fat accumulation and inflammation, and impaired liver function in broiler.

Heat stress adverse effects on respiratory system
Heat stress can cause damage to the lung tissue of broiler chickens by disrupting the integrity of the blood-air barrier and increasing permeability diseases can cause different degrees of lung damage Mammals mainly rely on sweat glands to dissipate heat and maintain body temperature balance (Yahav, 2015), but poultry lack sweat glands, so they primarily dissipate heat through respiration when the temperature is too high (Bell et al., 2001). High-frequency breathing leads to increased susceptibility of lung tissue damage in a heat stress environment. Damage to the blood-air barrier can lead to increased lung permeability, impaired oxygen and carbon dioxide exchange function, and induce respiratory difficulties (Wang et al., 2020), further leading to various lung diseases such as tuberculosis and pulmonary inflammation (Research has shown that heat stress causes lung injury and results in the upregulation of various proinflammatory cytokines, including tumor necrosis factor.

Conclusion
High ambient temperature has emerged as a major constraint for the future development of the poultry industry, especially in the tropics and subtropics. The scarcity of resources coupled with harsh environmental conditions is the most crucial predicaments in the way to rationalize optimum production of broiler. Heat stress disturbs the physiological biochemistry of the broiler which ultimately reduces feed intake and feed efficiency which ultimately results in reduced performance and productivity. Under hot environmental conditions, feed utilization is disturbed by the deposition of fat and oxidative stress. In addition, changes in blood cells, acid-base balance, immune response, liver health, and antioxidant status are some of the major dynamics altered by heat stress.

Alleviating the Adverse Effects of Heat Stress is mandatory to achieve Production & performance poultry Business.

Dr. Anvesha Bhan1, Dr. Sundus Gazal2 and Dr. Sabahat Gazal3
Division of Veterinary Microbiology and Immunology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu

The poultry sector is among the fastest-growing industries, playing a crucial role in providing employment, income, and animal protein to both urban and rural populations, while also serving as manure for crops. Despite the global increase in meat supply, challenges such as bird handling, housing, rearing, and disease control still hinder the industry’s progress. During the monsoon season, continuous rainfall can lead to higher relative humidity and lower temperatures, affecting both the quality and quantity of feed. Additionally, wind speed can influence disease outbreaks. These weather changes impact poultry production, particularly for laying birds, as egg production declines in extremely cold or hot weather. Such conditions stress the birds, compromising their immune systems and reducing their disease resistance. Some of the common poultry diseases during the rainy season are:

Fowl Pox: Fowl pox is a highly contagious disease affecting poultry birds of all ages, caused by a poxvirus transmitted mainly by mosquitoes and other blood-sucking insects. The prevalence of fowl pox increases during the wet season due to the abundance of stagnant water, which provides breeding grounds for mosquitoes. Additionally, wet litter from poorly shielded poultry houses can lead to fly problems. Fowl pox exhibits round lesions with scabby centers on the birds’ skin, primarily on the wattle, face, comb, and occasionally on the legs. It can also affect the mouth and windpipe, causing lesions that may block the throat and lead to suffocation. Lesions on the face can spread to the eyes, potentially causing temporary or permanent blindness.

Fowl Cholera: Fowl cholera is a bacterial disease caused by Pasteurella multocida, affecting birds aged 6 weeks and above. It is highly contagious with high mortality in acute cases. The bacterium spreads readily during the rainy season as wet litter harbors numerous microorganisms.In acute cases, birds may die suddenly without prior signs, while chronic cases show symptoms similar to fowl typhoid, including yellow, green, or grey diarrhea; loss of appetite; labored breathing; drooped wings and tail feathers; ruffled feathers; swelling of leg joints, sinuses, wattles, and footpads.

Salmonellosis, Colibacillosis, Pullorum Disease (Bacillary White Diarrhea): These bacterial diseases affect birds of all ages and thrive in farms with poor sanitation, especially when wet litter is left unchecked. They impact the digestive system, presenting symptoms such as severe diarrhea, loss of appetite, depression and emaciation, chicks suffering from omphalitis, white pasty diarrhea in pullorum disease, huddling together and labored breathing.

Aspergillosis: Aspergillosis, caused by Aspergillus fumigatus, is prevalent during the rainy season due to high humidity, which dampens feed and litter, creating a conducive environment for fungal growth. Inhalation of Aspergillus spores lead to respiratory issues and lesions in the lungs. It is exhibited as Acute form which is common in young chicks and is characterized by rapid onset and high mortality with symptoms like lethargy, depression, loss of appetite, difficulty breathing, and cyanosis; or as Chronic form which develops subtly over weeks or months and affects older birds with symptoms like weight loss, reduced appetite, respiratory issues, and changes in vocalization.

Coccidiosis: Coccidiosis, a parasitic disease caused by the protozoan Eimeria spp. is an intestinal infection which causes extensive intestinal damage. It is widespread in poultry and game birds during the rainy season where wet litter and high pen temperatures favour the sporulation of oocysts of the parasite. Clinical signs include bloody faeces, ruffled feathers, anaemia, somnolence, severe diarrhoea, and high mortality. Decreased growth, feed and water consumption, weight loss, and decreased egg production are common. Infected survivors may suffer long-term performance loss.

Managemental Practices in Monsoons: Achieving Maximum Efficiency
The monsoon season brings challenges such as high relative humidity and temperature fluctuations. These extreme weather conditions create a favourable environment for the propagation of various pathogenic organisms, including bacteria, viruses, fungi, parasites, and vectors like flies and mosquitoes. This necessitates careful consideration and appropriate measures to optimize bird health and ensure efficient production.

Housing Management for Poultry During Monsoon:
A well-maintained shed is crucial for minimizing climatic stress and health challenges in poultry. Before the monsoon season, it is important to inspect the roof and walls for any holes or leaks and repair them promptly. Ensure the drainage ditch around the shed is clear to prevent waterlogging. The roof should have side overhangs of at least 3 to 4 feet to prevent rainwater from entering the shed. Cover the side walls of the empty shed with polythene curtains that are in good condition and can be adjusted based on ammonia concentration or rain intensity. Improper curtain management can lead to poor ventilation, resulting in ammonia buildup, which can cause issues such as improper digestion, abnormal respiration, and a high incidence of ascites. During the day, allow 1-2 feet opening at the top of the side curtains to ventilate ammonia and other undesirable gases. Atleast a 10-feet perimeter outside the shed should be kept clean and free of bushes and grasses. Waterlogging in the surrounding area can lead to propagation of insects like mosquitoes and flies inside the shed and since these act as vectors for many infectious diseases, proper cleanliness and pest control becomes crucial. To control the insect population regular spray of insecticides like bleaching powder and formalin (3-5%) should be done.

Litter Management in Poultry Housing During Monsoon:
A good litter material absorbs moisture when the surface is moist and the air is humid, and releases moisture when the air is dry. Ideally, the litter moisture content should be between 25% and 30%. If moisture falls to around 20%, the litter becomes too dusty, and if it rises to around 40%, the litter becomes wet and caked, which is undesirable. There are various issues that are faced with poor litter management viz., wet and caked litter promotes rapid microbial growth, which may cause infections leading to irritation, cracking, and infection of the foot. High moisture content in litter leads to ammonia buildup in the poultry house. Ammonia and other noxious gases can damage the respiratory tract lining, exposing birds to infections. Although the maximum permissible level of ammonia in the litter is 25 ppm, but adverse reactions including irritation of the eyes and respiratory tract start appearing at concentrations as low as 6 ppm, while reduced animal performance may be observed at 11 ppm. Thus, the level of ammonia in the shed must be kept at the minimum.

1. Moisture Control:
– Regularly check litter moisture. Compress the litter sample in hand; if it shows crevices and gently falls apart, moisture is optimal. If it forms a cohesive ball, it is too wet. If it crumbles easily, it is too dry.
– If litter moisture exceeds 40%, it indicates wet and caked litter which requires immediate disposal and replacement with fresh litter.
– Practice litter racking twice a day to prevent caking.
– To reduce litter moisture, add 1 kg of slaked lime and 150 gm of bleaching powder per 100 ft² of floor area.
– Operate ceiling fans at a ratio of one fan per 300 birds in deep litter broiler farms.

2. Overall Maintenance:
– To prevent mold growth, treat new litter with a 2% aqueous solution of copper sulphate spray.
– Regularly inspect and maintain the poultry house roof and walls to prevent leaks and ensure good drainage around the shed.
– Use polythene curtains to cover side walls and adjust them based on ammonia concentration and rain intensity, allowing for proper ventilation.
– Maintain cleanliness around the shed, keeping at least a 10-foot perimeter free from bushes and grasses to prevent waterlogging and insect breeding.
– Use insecticides, bleaching powder, and formalin spray (3-5%) outside the shed to control insect populations.

Feed and Water management to navigate through the Monsoon Season

Feed Management:
1. Adjust diet formulations to include all vital nutrients, considering the reduced feed intake of the birds due to high temperature and humidity.
2. Avoid long-term storage of feed as shelf life is shorter due to high humidity.
3. Prevent feed from heating up or forming lumps, which indicate decomposition and mold growth.
4. Ensure that vehicles for feed transport are leak-proof and maintain a 4-5 day extra feed stock to avoid frequent transportation during rainy days.
5. Use a Dunnage system to store feed bags. Stack bags on wooden or bamboo pallets at least 1 foot off the floor and away from side walls to avoid moisture contact and allow air circulation.
6. Implement a FIFO (First In, First Out) system for feed distribution.
7. Avoid wooden feed troughs to prevent mold growth and toxin production. Use plastic troughs for easier cleaning and disinfection.
8. Clean the feeders daily with a dry cloth.

Water Management:
1. Ensure clean, safe water supply as it significantly impacts flock performance.
2. Regularly sanitize water to prevent contamination, especially during the rainy season when E. coli and other coliform counts are higher.
3. Use water sanitizers with sufficient contact time and proper dosing.
4. Acidify drinking water to lower the pH, which reduces bacterial growth. Drinking water pH should preferably be around 5.0 to 5.5 to inhibit most pathogens. Poultry prefer water with a pH of 6 to 6.8.
5. Clean drinkers daily with detergents and bleaching powder to reduce water-borne diseases.
6. Clean pipelines at least once a week to reduce biofilm formation.
7. Monitor Oxidation-Reduction Potential (ORP) to evaluate the effectiveness of water sanitizers. An ORP value > 650 mV indicates good quality water, which can be effectively sanitized with 2-4 ppm free chlorine.

Dr. Sekhar Basak
CMD, Innovista Group

At Innovista, we understand the paramount importance of maintaining optimal flock health and maximizing productivity in the poultry industry. To achieve this, we have developed a comprehensive approach known as integrated health management programs, which combine cutting-edge technologies, precision nutrition, and strategic disease management practices.

The cornerstone of an effective integrated health program is the synergistic combination of vaccinations and anticoccidial interventions. By employing these two elements harmoniously, we can provide comprehensive protection against the threat of coccidiosis, a parasitic disease that can severely impact bird health and performance.

Vaccinations play a pivotal role in stimulating the flock’s immune response, preparing the birds to mount an effective defense against coccidial infections. Simultaneously, the judicious use of anticoccidial products, such as ionophores or synthetic chemicals, helps to minimize the severity of coccidiosis outbreaks and mitigate the associated economic losses.

At Innovista, we recognize the invaluable expertise of veterinary professionals in implementing successful integrated health programs. Their in-depth knowledge and
experience allow them to design tailored vaccination protocols, guide the prudent use of anticoccidials, and continuously monitor flock health through regular observations and data analysis.

Effective integrated health programs rely on a data-driven approach, leveraging monitoring systems and analysis tools to gather insights into flock performance, environmental conditions, and potential health challenges. This enables proactive adjustments and optimizations to the program based on real-time information.

We believe that the journey to optimal flock health begins at the hatchery. By administering coccidial vaccines early in the birds’ lives, we can stimulate their immune
systems and establish a robust foundation for future disease resistance. This controlled exposure to coccidia facilitates the development of robust immunity, reducing the risk of disease outbreaks and economic losses down the line.

Building upon this foundation, we integrate in-feed anticoccidials into our integrated health programs, providing continuous protection against coccidiosis throughout the
production cycle. Our careful selection of ionophores or synthetic chemicals ensures effective control of coccidial infections while minimizing the impact on beneficial gut bacteria and promoting overall intestinal health.

At Innovista, we firmly believe that collaboration between our team of experts and poultry producers is the key to success. By combining our extensive knowledge, data-driven insights, and a commitment to best practices, we can tailor integrated health programs that optimize flock health, enhance productivity, and drive sustainable growth in the poultry industry.

Contact us at info@innovistaconsulting.com or +91 9871203111.

Wouter Van Der Veken, Global Product Manager Probiotics, Huvepharma

Dr Sanjay Singhal, Chief Operating Officer, Stallen South Asia Pvt. Ltd, Mumbai

Mycoplasma, contrary to many other organisms, lack a cell wall, making them smallest free-living organisms with respect to of both size and gene number. Pathogenic Mycoplasma species in chickens are Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS). MG is typically the more virulent species and results in substantial financial losses. On commercial layer farms across different age groups, MS is a prevalent pathogen and is more ubiquitous.

The ability of different strains of Mycoplasma synoviae (MS) to produce illness varies greatly, with numerous forms appearing moderate. In highly susceptible birds, more pathogenic MS strains can cause serious joint infections, respiratory illnesses, and reduced egg production.

MS often manifests as a mixed infection with other respiratory pathogens, which include the infectious bronchitis virus (IBV) and the Newcastle disease virus (NDV). MS may not necessarily be the primary the cause. These mixed infections can cause significant chronic respiratory illness, particularly under harsh environmental circumstances including high ammonia, low temperatures, and dust. Birds with MS may react more to other live vaccines. Layers of egg yolk peritonitis caused by E. coli have been linked to MS aetiology.

Transmission
Horizontal transmission occurs through direct contact. Birds carry the infection for the rest of their lives. In many respects, the spread appears to be like that of M. gallisepticum except that it is more rapid. Yet reports of slow spreading infections exist. Only a few percent of birds may show clinical symptoms, but most birds often acquire illness by respiratory transmission. Infection may also occur because of environmental contamination or fomites. In chickens and turkeys, vertical transmission is a crucial factor in the spread of MS. When commercial breeder flocks are infected during egg production, the rate of egg transmission seems to peak in the first 4-6 weeks following infection; beyond that, the transmission may stop, although the infected flock may shed at any moment.

Pathophysiology
The pathologic characteristics of synovitis induced by MS involve the joints’ synovial cells hypertrophy and become more proliferative. Activated synovial fibroblasts (SFs) are the primary constituents of hyperplastic synovial tissue in humans with arthritis and play a significant role in the pathophysiology of synovitis.

Matrix metalloproteinases, cathepsins, chemokines, and cytokines are produced by activated synovial fibroblasts, which worsen inflammation and degrade bone and cartilage. For arthritis, reducing the number of activated synovial fibroblasts is a potential treatment approach.

Clinical signs
In poultry, Mycoplasma synoviae usually manifests as upper respiratory tract infection; it may cause mild respiratory disturbances such as rales but is usually subclinical. When the infection spreads to the joints, certain strains of MS may cause a transition from the acute to the chronic phase. Exudative tenosynovitis, an inflammation of the tendons and synovial membranes brought on by invasion of the joint tissue, ultimately results in lameness. The keel bone bursa and the hock (tibial metatarsal) joints are the main regions affected; however other joints may also be damaged. Although this type can be observed in flocks as young as 4 weeks old, it usually manifests itself soon after mature pullets are transferred to the laying farm.

Generally, there is no impact on egg production if the flock is exposed to MS during the laying phase. Egg production may decrease, and desirable egg quality may decrease in flocks that face challenges throughout the laying season. A flock of MS-positive birds that are treated with periodic antibiotic feed therapy might display an irregular egg production curve. Due of restricted movement to feed, water, and nests, lameness from tenosynovitis might further affect egg production.

Oviduct tropism of MS strains have been found recently in commercial layers. It is noticed that flocks infected with certain strains of MS have a higher proportion of cracked and broken eggs. On the apex of the egg, or pointed end, there is a distinctive eggshell defect that may be seen. The rough surface of the eggshell, located 2 centimetres from the apex, is characterized by thinning and translucency, resembling glass eggs. These eggshells lack part of the palisade layer and the mammillary knob layer, according to scanning electron microscopy.

Diagnosis
Accurate diagnosis of mycoplasmosis is crucial for effective management. It is typically achieved through a combination of clinical signs, post-mortem examinations, and laboratory tests. These tests may include serology (blood tests), PCR (polymerase chain reaction), and bacterial isolation from affected tissues.

Treatment
Antibiotics can be administered to control the spread of the disease and manage clinical symptoms. Tetracyclines, tylosin, and lincomycin are commonly used antibiotics. However, it is important to note that these treatments are not curative and are used to suppress the disease.

Prevention and Control
Biosecurity Measures: Implement strict biosecurity measures to prevent the introduction and spread of mycoplasmosis. This includes limiting visitor access, maintaining separate footwear and clothing for workers, and disinfecting equipment and facilities regularly.
Cleanup Programs: Use of appropriate molecule for effective cleaning up of mycoplasmal infection prior to vaccination may provide better results.

Minimize Stress: Stress weakens the immune system, making birds more susceptible to infections. Provide a low-stress environment by ensuring proper nutrition, ventilation, and living conditions.

Surveillance: Regularly monitor your flock for any signs of illness. Early detection allows for prompt intervention and reduces the spread of the disease.

Vaccination: There are vaccines available for both MG and MS, Stallen has killed vaccines against both MG and MS named as MYC Vac and MS Vac respectively, which provides better protection against avian mycoplasmosis. Our MS Vac is the only killed vaccine against Mycoplasma synoviae available in Indian Market. Recommended dose of both vaccines by parenteral route 0.5ml/ bird.

For better results, proper cleanup program with effective anti- mycoplasmal drug is recommended. The above-mentioned vaccines can also be used in midlay vaccination if the priming is done with the live vaccines.

Recommended vaccination schedule

Water Hygiene Challenges and Management in Commercial Poultry Farming during Summer Season

Dr Davendar Singh Kalwani, Technical Sales Manager, Intracare SEA Pvt Ltd

Introduction:
Summer season brings with it extreme challenges for the poultry industry. Among all the prevailing issues, water hygiene remains the top priority, as far as poultry production is concerned. Quality of water will in general, have a direct bearing on poultry’s health and production. Good quality water is important for poultry’s growth, reproductive performance, and general well-being. The prevailing high temperatures coupled with an increased microbial activity during the summers obviously make it tough to maintain the desirable standards of water hygiene. This article attempts to understand the risks involved and the strategies to manage the water hygiene in this summer in a better way. The article also tries to identify the factors contributing to waterborne microbial contamination and understand the impacts of water contamination on poultry health and welfare. Awareness of the peculiar dynamics of summer management, in terms of water hygiene, can help farmers in preventing some of the losses that are usually suffered by them during the summer and throughout the year.

Impact of summer season on water quality:
During summer, various environmental factors can affect the quality of water. The rise in temperature of water is the most important factor. As the water temperature increases, it creates optimal conditions for microbial growth. Mesophilic bacteria including major pathogens proliferate rapidly in such conditions, hence increasing the risk of water contamination in poultry production. These microbes might lead to severe illnesses and reduced performance in terms of growth and reproduction.

Moreover, the elevated water temperature accelerates the decomposition of organic matter which serves as a nutrient source for various microorganisms. Due to this rapid decomposition in warmer temperatures, the level of nutrients, such as nitrogen and phosphorus in water increases along with release of dissolved solids which further alters the water composition negatively.

Additionally, this can fuel the algal bloom in underground water reservoirs. Some species of algae produce toxins that are harmful to both animals and humans if ingested. Also, as algae die and decomposes it hastens the degradation of water quality.

In addition to microbial contamination and algal blooms, summer conditions can also aggravate other water quality issues in poultry operations. Reduced rainfall and drought conditions in certain regions can result in lower water levels in reservoirs and water bodies. Lower water levels concentrate pollutants, such as nutrients, chemicals, and sediment, leading to higher concentrations in the remaining water. This can further degrade water quality and increase the risk of contamination for poultry.

Biofilm as a hidden threat:
Formation of biofilm during summer is another crucial aspect involved in degrading water quality particularly in water pipelines. Biofilms is a slimy layer consisting of complex communities of microbes that attach to surfaces of water pipes, tanks, and drinkers. Warm climate can enhance the growth and proliferation of various bacteria, easing the formation of such biofilms. These biofilms pose several challenges to the quality of water and poultry health. It provides protection for microbes inside it by shielding them from disinfectants and making them more resistant to removal, this allows pathogens to persist in the water systems for long durations making itself a source of infection.

Biofilms can also cause deterioration of water infrastructure; its accumulation might lead to corrosion of pipes and fittings which can compromise the integrity of water distribution system. Additionally, it can cause blockages and reduce the flow of water and thus affecting water flow to drinkers which can lead to dehydration in birds.

Furthermore, biofilms act as a reservoir for pathogens, releasing them into the water intermittently and perpetuating the cycle of contamination. This can pose a continuous threat to poultry health, increasing the likelihood of disease outbreaks and impacting the overall productivity of the operation.

Effects of poor water quality on poultry production:
1. Biofilm inside water pipeline may reduce intake, causing dehydration and poor growth.
2. Biofilms can release pathogens, affecting bird health and productivity.
3. Contaminants may lead to digestive issues, diarrhoea, and poor growth.
4. Poor quality of water can affect egg quality resulting in thin shelled eggs and reduced hatchability in fertile eggs.
5. Stress from poor water quality drops reproductive performance in poultry flocks.
6. Mortality rates can increase due to stress, dehydration, and disease susceptibility.
7. Water contaminants compromise vaccine efficacy, leaving birds vulnerable to infections.
8. It might worsen the effect of concurrent viral or any other diseases.
9. Clogged delivery systems can hamper vaccine administration, risking inadequate immunity in poultry.
10. It can increase the chances of vertical transmission of bacterial diseases in progeny.

Management Strategies for Summer Water Hygiene:
Following strategies may be followed to ensure quality drinking water to poultry birds:
1. Regularly clean the water sources, pipes, and drinkers to prevent biofilm and pathogen buildup.
2. Test the water quality regularly for pH, TDS, and microbial contamination.
3. Use of good quality water disinfectant and sanitizers and follow manufacturer guidelines.
4. Control water temperature to prevent microbial growth.
5. Minimize water wastage by fixing leaks and optimizing delivery systems.
6. Educate farm staff on water hygiene.
7. Maintain records of cleaning schedules and water quality tests.

Ensuring water quality at poultry farms:
Along with all the management strategies, the most crucial step is pipeline cleaning and water sanitation. There are many chemical agents available for the same purpose. Choosing the best water sanitizer and cleaning agent should be based on several characteristics.
When it comes to pipeline cleaning methods, the following characteristics are desirable:
1. Efficiency: The cleaning method should effectively remove biofilms, mineral deposits, sediment, and other contaminants from water pipelines to maintain optimal water quality and flow rates.
2. Non-Corrosive: Cleaning agents or procedures should not corrode or damage pipeline materials, ensuring the longevity and integrity of the water distribution system.
3. Accessibility: Pipeline cleaning methods should be accessible and practical for poultry producers, whether through manual cleaning procedures or automated cleaning systems.
4. Frequency: The cleaning frequency should be appropriate to prevent biofilm formation and ensure consistent water quality for poultry health and performance.
5. Validation: Cleaning procedures should be validated to confirm their effectiveness in removing contaminants and maintaining water sanitation standards.

When considering water sanitizers for poultry operations, several characteristics are essential to ensure effective and safe water management:
1. Broad-Spectrum Activity: An ideal water sanitizer should have broad-spectrum activity against a wide range of bacteria, viruses, fungi, and other pathogens commonly found in poultry drinking water. This ensures comprehensive protection against disease-causing organisms.
2. Non-Toxic and Safe: The sanitizer should be non-toxic to poultry and humans when used at recommended concentrations. It should not leave harmful residues that could affect bird health or compromise food safety.
3. Residue-Free: After application, the sanitizer should degrade into non-toxic by-products or dissipate without leaving any harmful residues in the water or water distribution system.
4. Stability: The sanitizer should remain stable under varying environmental conditions, including temperature fluctuations and water pH levels, to maintain its effectiveness over time.
5. Compatibility: It should be compatible with commonly used materials in poultry water systems, such as PVC, polyethylene, and stainless steel, to prevent corrosion or damage to pipelines and water equipment.
6. Ease of Application: The sanitizer should be easy to apply and should not require complex equipment or procedures for effective use. This ensures practicality and efficiency in poultry farm operations.
7. Regulatory Compliance: The sanitizer should comply with regulatory standards and guidelines set forth by relevant authorities, ensuring its safety and efficacy for use in poultry production.
8. Environmental Impact: Consideration should be given to the environmental impact of the sanitizer, including its biodegradability and potential effects on water quality in surrounding ecosystems.

Based on these characteristics, selecting a suitable option is very perplexing. In general, quaternary ammonium salts (commonly called quats) and hydrogen peroxide fulfil almost all the requirements but they have some drawbacks as well. Hydrogen peroxide is an unstable compound and loses its efficacy in very short period making it difficult to get uniform results across pipeline. Quats are effective for water sanitation, but they have limited action on biofilms particularly mature ones. However, 50% stabilized hydrogen peroxide is an excellent choice as it easily overcomes the above problems. Its broad-spectrum effectiveness, safety, non-corrosive properties, long shelf-life, and environmental compatibility make it an indispensable tool in safeguarding the health and profitability of poultry flocks, particularly in the challenging conditions of the summer season.

Conclusion
In conclusion, managing water hygiene effectively is among top priority for commercial poultry farmers, especially during the challenging conditions of summer. Summer’s heat and increased microbial activity threaten water quality. Regular cleaning, disinfection, temperature control, and water testing can help combat these threats successfully. Minimizing water wastage waste, staff training, and record-keeping further strengthen water hygiene plans. By proactively managing water, poultry farmers ensure the long-term health and profitability of their flocks.

It was first discovered in the juice of sugar beets. Naturally accumulated in plants as osmolyte to protect against salt and temperature stress. Derivative of glycine (amino acid). Neutral molecule with bipolar structure (zwitterion) as shown in Fig. 1 contains three methyl groups.

Fig.1: Chemical Structure of Betaine

2. Betaine functions as (mode of action):
A. Methyl donor – methyl groups used for protein synthesis and other metabolic processes. Methyl groups play a pivotal role in several cellular processes, including DNA methylation, synthesis of phosphatidylcholine, and protein synthesis. Choline and betaine are both capable of donating methyl groups. However, for choline to do so, it must first be converted into betaine as shown in Fig. 2. In poultry, the capacity to synthesize betaine from choline is limited, thus making dietary supplementation the primary source.

Fig. 2: Role of betaine in the methionine cycle in liver

Betaine can substitute for choline in performing the following functions:
1) Regulating fat metabolism in the liver to prevent abnormal fat accumulation in hepatocytes.
2) Serving as a methyl donor for the formation of methionine and creatine, through its involvement in the transmethylation pathway.
Betaine cannot replace choline in the function of maintaining cell membrane and structure as an emulsifier to transport lipids, since choline is a constituent of phospholipids. Similarly, betaine cannot replace choline as a precursor of acetylcholine in the transmission of nerve impulses.

B. Osmo-regulator: – ability to bind and retain water in a reversible manner.
Osmolytes are compounds that aid in the regulation of osmotic pressure within cells and tissues, playing a crucial role in preserving cellular integrity.
Dehydration, disease, heat stress, and other factors can cause alterations in the water content of cells. Osmolytes can be either inorganic ions such as Na+, K+, Cl-, or organic compounds such as amino acids, certain sugars, and betaine. Betaine plays a crucial role in stabilizing cellular metabolic function during periods of stress, preserving the cell’s capacity to uptake nutrients, unlike osmolytes such as Na+, K+, and Cl-. Moreover, it offers protection to intracellular enzymes against osmotic inactivation.

3. Heat stress
Heat stress is a major challenge in poultry production, especially during the hot summer months. It occurs when birds face difficulty in achieving a balance between body heat produced and heat loss. This imbalance can lead to several health issues and production losses.

4. The Role of Betaine in Enhancing Poultry Health During Heat Stress.
a) Betaine aids in preserving intestinal integrity by facilitating water retention, increasing cell volume, promoting anabolic activity, and maintaining cellular integrity as shown in fig. 4. which are Representative photomicrographs of the ileum after 10 days of the experiment from broilers fed a control diet (CON, A and C) and betaine (BET, B and D) on villous height under thermoneutral (TN, A and B) or after 10 days being exposed to heat stress (HS, C and D).

Fig. 3 – Intestinal barrier damage in HS (Soheil Varasteh, et al. Nutrients, 2020)

Fig. 4 – Impact of betaine on intestinal integrity of broiler birds in Heat stress conditions (Shakeri et al, Animals 2020)

b) Betaine has three methyl groups in its structure and donates them in various metabolic reactions, which can spare compounds like methionine, choline, and folic acid. Therefore, supplementing with betaine may reduce the need for these nutrients.

c) The growth rate of poultry birds is enhanced by betaine, which conserves energy that would otherwise be expended on the Na+/K+ pump and Calcium pump in high temperatures. This conserved energy can then be directed towards growth.

d) Betaine enhances the concentration of beneficial short-chain fatty acids, such as acetic and propionic acid, which are vital to host bacteria like Lactobacillus and Bifidobacterium in poultry. This improvement enables these bacteria to effectively inhabit the caecum and inhibit the colonization of harmful bacteria in the intestinal tract.

e) Betaine supplementation in laying hens leads to an increase in daily egg mass production, reduces thin eggshell issues which are related to heat stress, and helps to enhance serum concentrations of estradiol and melatonin.

f) Trouw Nutrition’s Betaine is proven to elevate production performance even under heat stress conditions, notably increasing breast meat percentage through the provision of essential methyl groups, as depicted in Fig. 5. Recognizing that high-performing animals demand superior nutrition for sustained health and optimal growth, Selko Feed Additives introduces TNIbetain. This meticulously tested supplement supports animal performance across multiple metabolic pathways. TNIbetain adheres strictly to the stringent quality standards upheld by Trouw Nutrition Feed Additives.

Fig. 5: Effect of Trouw Nutrition betaine on broiler performance
Contrasting the Attributes of Trouw Nutrition’s Natural Betaine with Synthetic Betaine

Recommended Dosage:
For broiler, layer, and breeder birds: 0.5 to 1 kg per ton of feed. However, in challenging conditions such as heat stress, the Betaine dosage can be increased to up to 2 kg per ton of feed.
g) Betaine has been found to significantly enhance hematological parameters, including RBC and platelet count, while reducing the number of heterophils and increasing the number of lymphocytes. The reduction in lymphocyte count during heat stress is attributed to the rise in inflammatory cytokines, which stimulate hypothalamic production of corticotrophin releasing hormones.
h) Betaine aids in the expansion of intestinal mucosa, thereby enhancing the absorption and utilization of nutrients, which results in improved digestibility of crude protein, crude fiber, ether extract.
i) Studies have demonstrated that betaine interacts with lipid metabolism by promoting the oxidative catabolism of fatty acids through its involvement in carnitine synthesis. Therefore, betaine can be utilized to increase the proportion of lean meat and reduce fat in poultry carcasses.
j) Betaine acts as an osmoregulatory in the intestine, optimizing water and salt balance within cells for efficient nutrient absorption and reducing litter moisture. It increases villus height, protecting enterocytes during challenges like coccidiosis, and strengthens the gut, reducing damage during infections as shown in Fig. A, B and C.
The various effects described above are either directly or indirectly linked to betaine’s osmoregulatory function and its role in methionine biosynthesis.
Betaine emerges as a pivotal component in poultry health management, particularly in the face of heat stress challenges. Originating from sugar beets, its molecular structure rich in methyl groups facilitates its dual function as a methyl donor and osmoregulator, essential for maintaining cellular integrity and supporting metabolic processes. Amidst heat stress conditions, Betaine supplementation showcases remarkable efficacy, preserving intestinal integrity, conserving energy expenditure, and enhancing production performance. Its multifaceted benefits extend to improvements in hematological parameters, nutrient absorption, and lipid metabolism. With its proven effectiveness and adherence to stringent quality standards, Betaine stands as a crucial asset in optimizing poultry health and performance under challenging environmental conditions, exemplifying the potential of innovative nutritional strategies in safeguarding livestock welfare and productivity.

For further information, kindly write to us at customercareindia@trouwnutrition.com or visit our website: www.trouwnutrition.in

Introduction
Avian mycoplasmosis was primarily described in turkeys in 1905 and in chickens in 1930. There are 23 named species of mycoplasma recovered from avian sources but only two of them are established pathogens for domestic poultry as Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS) causes ‘Chronic Respiratory Disease’. Mycoplasma pathogens cause upper respiratory and locomotory illness in chickens and other avian species. They are responsible not only for clinical diseases but also for decreased weight gain, lowered feed conversion efficiency, reduced hatchability, and downgrading at slaughter (Bradbury, 2001).

Mycoplasma gallisepticum (MG) infection in the commercial poultry industry is common in many areas. Despite the great efforts by poultry breeding companies made towards eradication of pathogenic mycoplasmas from poultry flocks, Still Mycoplasma gallisepticum infection is of continuing economic concern in commercial broiler breeder chicken flocks. Failure in eliminating the disease in grandparent (GP) stock, it persists in broiler breeders and broilers through vertical transmission. The continued presence of MG in commercial broiler breeder flocks suggests that efforts at eradication were not highly successful. This organism is smaller than common bacteria and larger than viruses, but lacks a cell wall. This characteristic makes MG extremely fragile (no cell wall) and difficult to culture (specialised growth requirement) and host adapted (avian only).
Respiratory tract infections are of great importance in poultry industry, causing heavy economic losses. Mycoplasma gallisepticum and Mycoplasma synoviae are the most pathogenic organisms of the respiratory tract.

Other respiratory tract infections include both viral pathogens (Newcastle disease virus, Infectious bronchitis virus, avian influenza virus) and bacterial pathogens (Salmonella pullorum, Escherichia coli, Avibacterium paragallinarum, etc) cause disease independently and in association with each other and causes Complex Chronic Respiratory Disease (CCRD).

Mycoplasma control for any companies requires integrated approach involving diligent biosecurity, animal husbandry & disease survivallance. The consequences of wide spread infection in breeder operation can be devastating result of both as direct and indirect losses occurring throughout the production cycle (Ley,2003).

Transmission
MG and MS can spread through horizontally and vertically route of susceptible birds with infected chickens; spread may also occur by contaminated airborne dust, droplets, or feathers (Ley and Yoder, 1997). It can be transmitted through the chicken hatching egg to the offspring. MG has been isolated from the oviduct of infected chickens and semen of infected roosters (Yoder and Hofstad, 1964).

Clinical Sign
Both diseases are economically important, egg transmitted, and hatchery disseminated diseases. They lead to tremendous economic losses in poultry production as a result of decreased hatchability and egg production, reduced quality of day-old chicks, reduced growth rate. Chicken showed swelling of the facial skin, and the eyelids, increased lacrimation, congestion of conjunctival vessels, and respiratory rales.

Mycoplasma synoviae (Ms) infection is usually known as infectious synovitis, an acute-to-chronic infectious disease for chickens involving primarily the synovial membranes of joints and tendons sheaths. However, during recent years, MS has less frequently been associated with synovitis but more frequently associated with airsacculitis in chicken.

Pathogenesis
It is presumed that MG enters the respiratory tract by inhalation of aerosols or via the conjunctiva and attaches to mucosal cells by its well-organized terminal organelles, which remains and spread in respiratory system.

As MG & MS are exhibiting with no cell wall, it is readily killed by most of the disinfectants, heat, and sunlight, and does not survive for prolonged periods outside the host. MG can remain viable
1. Chicken faeces for 1-3 days at 20°C,
2. Muslin cloth 3 days at 20°C or 1 day at 37°C,
3. In egg yolk 18 week at 37°C or 6 week at 20°C.

It only remains viable in the environment, outside the chicken, for typically up to 3 days. For this reason, MG is fairly easy to eliminate on single age, all-in all-out poultry farms Since MG can be transmitted vertically. Establishing the MG-clean status of breeder flocks and maintaining that status can be accomplished by participation in control programmes. An MG eradication programme may be initiated by treatment of breeders and their hatching eggs to reduce egg transmission. Attempts to eliminate egg transmission of MG by medication of breeder flocks or their progeny with antimycoplasmal prevention drug have generally been able to produce considerable reduction in rate of MG infection but generally were not adequate to obtain entirely infection-free flocks. Previously successful methods were the treatment of hatching eggs with heat and/or antimycoplasmal. For heat treatment eggs were gradually heated in a forced-air incubator to reach an internal temperature of 46.10C over 12-14 hour and then allowed to return to room temperature (Yoder, 1970). Hatchability was sometimes reduced 8-12%, but MG and MS appeared to be inactivated. Egg dipping with a temperature or pressure differential has been used by several researchers as a means of getting antibiotics into hatching eggs to eliminate egg transmitted MG (Alls et al., 1963;Hall et al., 1963; Stuart and Bruins, 1963).

Losses Can Occurs as Result of
1. Decreased egg production
2. Decreases egg hatchability
3. Decreased day old chick quality and chick viability
4. Increase chick mortality
5. Higher FCR and low weight gain
6. Costly control measures involving biosecurity, vaccination & medication.

Control of pathogenic avian mycoplasma can consist of one of three general approaches, according to Kleven (2008): The mycoplasma infection are transmitted both horizontally and vertically and it’s remained in the flock constantly as sub clinical form. To control MG infection in broiler breeder, laying hens and commercial broilers chicken the major specific focus is given on vaccination and medication.

1. Maintenance of Flocks, which are Free of Infection.
To keep a flock free of infection is difficult, especially in areas where large populations of chickens have grown up, as the industry has expanded. To maintain freedom from mycoplasma requires a mycoplasma free source, on a single age, ‘all in all out’ site, with good biosecurity and an effective monitoring system.

2. Control by Vaccines
The use of mycoplasma vaccines in breeding & laying hens has grown over recent years to reduce the impact of infections, but these can confuse the usual serological monitoring systems. They may control an infection in the chicken clinically but there is still a potential risk of vertical transmission to the egg and chick. Vaccination could not completely prevent the occurrence of EAA, although a significant reduction of EAA egg production (approximately 50%) was recorded. Moreover, a delay in the onset of egg production was observed in the vaccinated birds (Feberwee et.al. 2009).

1. Killed/Inactivated Vaccines
– These are M. gallisepticum killed organisms with oil emulsion adjuvants to protect the birds from infection with virulent M. gallisepticum.
– Several adjuvant enhanced bacterin vaccines but they are expensive and administration is difficult because they need to be injected twice with a 4-6 week interval (Ley, 2003).
– Killed vaccines have been shown to reduce, but not eliminate the M. gallisepticum infection and are not effective in long term control of infection in multiple age farms.
Killed vaccination did not reduce horizontal spread of M. gallisepticum (Levisohn et. al.,2000).
– These are more stable and safer than live vaccine.

2. Live/Attenuated Vaccine
There is three type of live vaccines is available for M. gallisepticum viz.
A. Connecticut F-Strain
B. MG 6/85 Strain
C. TS-11 Strain (temperature Sensitive Mutants)

A. Connecticut F-Strain
– Live F-strain M. gallisepticum vaccine is a relatively mild strain that originate from the Connecticut F strain of United States. Despite the advantages of the f-strain vaccine it has many of the disadvantages of the inactivated vaccines.
– MG free chickens tend to lay better than F-strain immunised ones.
– F-strain is too virulent for young chicks.
– F-strain is capable of lateral spread in the flock.
– F-strain does not completely block trans ovarian transmission when birds are challenged with virulent MG.

B. MG 6/85 STRAIN
– The 6/85 strain of MG is in lyophilised form and originate from United States.
– It has low virulence in chicken.
– Vaccinates were protected against airsacculitis and colonisation of the trachea was detectable from 4 to 8 weeks after vaccination (Ley, et. al., 1997).

C. TS-11 STRAIN
– ts-11 is a live chemically induced mutant strain of MG is in frozen form and developed from Australian MG field isolate (Whithear et. al.,1990a).

3. Control by Special Antibiotics
Medication of a flock but can prevent subsequent losses in breeders & laying hens. MS Infections could be treated with antimicrobial use in breeders, layers flock and eggs to prevent vertical transmission.
Control of MG and MS infection in broiler chicken by medication is the most practical way to minimize the transmission of disease and economic losses.
– The most important macrolide agent used for treatment and control of mycoplasma infection is Tilmicosin Phosphate.
– Tilmicosin is a broad-spectrum bacteriostatic synthesized from tylosin molecule which is having 75 percent more intra alveolar concentration in the lungs tissue to work efficiently against mycoplasma as organism remain intracellular in the cell and tissue.

In Broiler Breeder & Commercial Layers
– It is very important to treat chicks from day first of life to combat against mycoplasma, Tilmicosin Phospate-25% @ dose rate of 15-20mg/kg body weight through drinking water for 3 successive days every 5 weeks up to for 16th to 20th weeks.

– After 20th or 24th week incorporate Tiamulin through feed as per recommendation of veterinarian.

– It is emphasized to follow best antimycoplasmal drug prevention programme through feed.

In Commercial Broilers
– It is suggested to use Tylosin Tartarate 100% through drinking water for first 3 days @ dose rate of 65 mg/kg of BW.

– In high risk or known source of infected breeders it is suggested to use Tilmicosin Phosphate-25% through drinking water for first 3 days @ dose rate of 15mg/kg of BW.

The medication can be repeated on a monthly, three weekly or two weekly basis depending on the mycoplasma status of the flock or the ‘risk’ of breakdown from the proximity of infected neighbours.